Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/36202
Title: Immunological biomarkers of subclinical infection in household contacts of leprosy patients
Authors: Queiroz, Edson A.
Medeiros, Nayara Ingrid
Mattos, Rafael T.
Carvalho, Ana Paula M.
Alves, Marina L. Rodrigues
Dutra, Walderez Ornelas
Lana, Francisco C. Félix
Gomes, Juliana A. S.
Oliveira, Rodrigo Correa de
Affilliation: Universidade Federal de Minas Gerais. Escola de Enfermagem. Belo Horizonte, MG, Brasil.
Fundação Oswaldo Cruz. Instituto René Rachou. Imunologia Celular e Molecular. Belo Horizonte, MG, Brasil / Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Morfologia. Laboratório de Biologia das Interações Celulares. Belo Horizonte, MG, Brasil.
Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Morfologia. Laboratório de Biologia das Interações Celulares. Belo Horizonte, MG, Brasil.
Universidade Federal de Minas Gerais. Escola de Enfermagem. Belo Horizonte, MG, Brasil.
Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Morfologia. Laboratório de Biologia das Interações Celulares. Belo Horizonte, MG, Brasil.
Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Morfologia. Laboratório de Biologia das Interações Celulares. Belo Horizonte, MG, Brasil.
Universidade Federal de Minas Gerais. Escola de Enfermagem. Belo Horizonte, MG, Brasil.
Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Morfologia. Laboratório de Biologia das Interações Celulares. Belo Horizonte, MG, Brasil.
Fundação Oswaldo Cruz. Instituto René Rachou. Imunologia Celular e Molecular. Belo Horizonte, MG, Brasil / Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Morfologia. Laboratório de Biologia das Interações Celulares. Belo Horizonte, MG, Brasil.
Abstract: Hansen's disease (or leprosy) still persists as a serious public health issue. Its diagnosis is based primarily on the detection of clinical signs that are characteristic of the disease. Studies have pointed to the selection of a set of serological and cellular biomarkers of subclinical infection that result in an efficient diagnosis. The aim of this study was compare index cases and their household contacts to identify differentially expressed biomarkers of immune response in leprosy that could provide reliable evidence of subclinical infection in household contacts. The study population consisted of index cases with multibacillary form (IC, n = 13) and their household contacts (HC, n = 14). Serum cytokines and chemokines were quantified using the cytometric beads array (CBA) system. The humoral response was assessed by ELISA test. Flow cytometry was used to characterize the cellular immune response. Monocyte and CD4 + T lymphocytes frequency was significantly higher in IC. Both CD4+ and CD8 + T lymphocytes had a reduced CD25 expression in HC. The immunoglobulin (Ig)M profile anti- NDO-HSA, LID-1, and NDOLID antigens was significantly higher in IC. This study points to the monocyte and CD4+ lymphocyte frequency, as well as specific IgM profile, as predictors of subclinical infection in the household contacts.
Keywords: Hansen’s disease
Leprosy
Household contact
Subclinical infection
Immunological
Biomarkers
Issue Date: 2019
Publisher: Elsevier
Citation: QUEIROZ, Edson A. et al. Immunological biomarkers of subclinical infection in household contacts of leprosy patients. Immunobiology, v. 224, n. 4, p. 518-525, 2019.
DOI: 10.1016/j.imbio.2019.05.002
ISSN: 0171-2985
Copyright: restricted access
Appears in Collections:MG - IRR - Artigos de Periódicos

Files in This Item:
File Description SizeFormat 
Immunological biomarkers.pdf8.85 MBAdobe PDF    Request a copy



FacebookTwitterDeliciousLinkedInGoogle BookmarksBibTex Format mendeley Endnote DiggMySpace

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.