Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/36211
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dc.contributor.authorEsteves, Eliane
dc.contributor.authorBizzarro, Bruna
dc.contributor.authorCosta, Francisco Borges
dc.contributor.authorRamírez-Hernández, Alejandro
dc.contributor.authorPeti, Ana Paula Ferranti
dc.contributor.authorCataneo, Allan Henrique Depieri
dc.contributor.authorWowk, Pryscilla Fanini
dc.contributor.authorTimóteo, Rodolfo Pessato
dc.contributor.authorLabruna, Marcelo Bahia
dc.contributor.authorSilva Junior, Pedro Ismael
dc.contributor.authorSilva, Célio Lopes
dc.contributor.authorFaccioli, Lúcia Helena
dc.contributor.authorFogaça, Andréa Cristina
dc.contributor.authorSorgi, Carlos Arterio
dc.contributor.authorSá-Nunes, Anderson
dc.date.accessioned2019-10-04T20:02:50Z
dc.date.available2019-10-04T20:02:50Z
dc.date.issued2019
dc.identifier.citationESTEVES, Eliane et al. Amblyomma sculptum Salivary PGE2 Modulates the Dendritic Cell-Rickettsia rickettsii Interactions in vitro and in vivo. Frontiers in Immunology, v. 10, n. 118, p. 1-15, 2019.
dc.identifier.issn1664-3224
dc.identifier.urihttps://www.arca.fiocruz.br/handle/icict/36211
dc.language.isoeng
dc.publisherFrontiers Media
dc.rightsopen access
dc.subject.otherCarrapato Estrela
dc.subject.otherSaliva
dc.subject.otherPGE2
dc.subject.otherImunomodulação
dc.titleAmblyomma sculptum Salivary PGE2 Modulates the Dendritic Cell-Rickettsia rickettsii Interactions in vitro and in vivo
dc.typeArticle
dc.identifier.doi10.3389/fimmu.2019.00118
dc.description.abstractenAmblyomma sculptum is an important vector of Rickettsia rickettsii, causative agent of Rocky Mountain spotted fever and the most lethal tick-borne pathogen affecting humans. To feed on the vertebrate host's blood, A. sculptum secretes a salivary mixture, which may interact with skin resident dendritic cells (DCs) and modulate their function. The present work was aimed at depicting the A. sculptum saliva-host DC network and the biochemical nature of the immunomodulatory component(s) involved in this interface. A. sculptum saliva inhibits the production of inflammatory cytokines by murine DCs stimulated with LPS. The fractionation of the low molecular weight salivary content by reversed-phase chromatography revealed active fractions eluting from 49 to 55% of the acetonitrile gradient. Previous studies suggested that this pattern of elution matches with that observed for prostaglandin E2 (PGE2) and the molecular identity of this lipid mediator was unambiguously confirmed by a new high-resolution mass spectrometry methodology. A productive infection of murine DCs by R. rickettsii was demonstrated for the first time leading to proinflammatory cytokine production that was inhibited by both A. sculptum saliva and PGE2, a result also achieved with human DCs. The adoptive transfer of murine DCs incubated with R. rickettsii followed by treatment with A. sculptum saliva or PGE2 did not change the cytokine profile associated to cellular recall responses while IgG2a-specific antibodies were decreased in the serum of these mice. Together, these findings emphasize the role of PGE2 as a universal immunomodulator of tick saliva. In addition, it contributes to new approaches to explore R. rickettsii-DC interactions both in vitro and in vivo.
dc.creator.affilliationUniversidade de São Paulo. Instituto de Ciências Biomédicas. Departamento de Imunologia. São Paulo, SP, Brasil.
dc.creator.affilliationUniversidade de São Paulo. Instituto de Ciências Biomédicas. Departamento de Imunologia. São Paulo, SP, Brasil.
dc.creator.affilliationUniversidade de São Paulo. Faculdade de Medicina Veterinária e Zootecnia. São Paulo, SP, Brasil.
dc.creator.affilliationUniversidade de São Paulo. Faculdade de Medicina Veterinária e Zootecnia. São Paulo, SP, Brasil.
dc.creator.affilliationUniversidade de São Paulo. Faculdade de Ciências Farmacêuticas de Ribeirão Preto. Departamento de Análises Clínicas, Toxicológicas e Bromatológicas. Ribeirão Preto, SP, Brasil.
dc.creator.affilliationFundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Virologia Molecular. Curitiba, PR, Brasil.
dc.creator.affilliationFundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Virologia Molecular. Curitiba, PR, Brasil.
dc.creator.affilliationUniversidade Federal do Triângulo Mineiro. Instituto de Ciências Biológicas e Naturais. Uberaba, MG, Brasil.
dc.creator.affilliationUniversidade de São Paulo. Faculdade de Medicina Veterinária e Zootecnia. São Paulo, SP, Brasil.
dc.creator.affilliationInstituto Buntantan. Laboratório Especial de Toxinologia Aplicada. São Paulo, SP, Brasil.
dc.creator.affilliationUniversidade de São Paulo. Faculdade de Medicina de Ribeirão Preto. Departamento de Bioquímica e Imunologia. Ribeirão Preto, SP, Brasil.
dc.creator.affilliationUniversidade de São Paulo. Faculdade de Ciências Farmacêuticas de Ribeirão Preto. Departamento de Análises Clínicas, Toxicológicas e Bromatológicas. Ribeirão Preto, SP, Brasil.
dc.creator.affilliationUniversidade de São Paulo. Instituto de Ciências Biomédicas. Departamento de Parasitologia. São Paulo, SP, Brasil / Instituto Nacional de Ciência e Tecnologia em Entomologia Molecular / Conselho Nacional de Desenvolvimento Científico e Tecnológico. Rio de Janeiro, RJ, Brasil.
dc.creator.affilliationUniversidade de São Paulo. Faculdade de Ciências Farmacêuticas de Ribeirão Preto. Departamento de Análises Clínicas, Toxicológicas e Bromatológicas. Ribeirão Preto, SP, Brasil.
dc.creator.affilliationUniversidade de São Paulo. Instituto de Ciências Biomédicas. Departamento de Imunologia. São Paulo, SP, Brasil / Instituto Nacional de Ciência e Tecnologia em Entomologia Molecular / Conselho Nacional de Desenvolvimento Científico e Tecnológico. Rio de Janeiro, RJ, Brasil.
dc.subject.enAmblyomma sculptum
dc.subject.enTicks
dc.subject.enDinoprostone
dc.subject.enDendritic Cells
dc.subject.enImmunomodulation
dc.subject.esGarrapatas
dc.subject.esInmunomodulación
dc.subject.decsCarrapatos
dc.subject.decsDinoprostona
dc.subject.decsCélulas Dendríticas
dc.subject.decsRickettsia rickettsii
dc.identifier.eissn1664-3224
Appears in Collections:PR - ICC - Artigos de Periódicos

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