Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/36398
Title: Detection of anti-Trypanosoma cruzi antibodies by chimeric antigens in chronic Chagas disease-individuals from endemic South American countries
Authors: Del-Rei, Rodrigo Pimenta
Leony, Leonardo Maia
Celedon, Paola Alejandra Fiorani
Zanchin, Nilson Ivo Tonin
Reis, Mitermayer Galvão dos
Gomes, Yara de Miranda
Schijman, Alejandro Gabriel
Longhi, Silvia Andrea
Santos, Fred Luciano Neves
Affilliation: Faculdade de Tecnologia e Ciências da Bahia. Salvador, BA, Brasil.
Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil.
Instituto de Biologia Molecular do Paraná. Curitiba, PR, Brasil.
Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil.
Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil. / Universidade Federal da Bahia. Departamento de Patologia e Medicina Legal. Salvador, BA, Brasil. / Department of Epidemiology of Microbial Diseases. School of Public Health. Yale University. New Haven, Connecticut, United States of America.
Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Recife, PE, Brasil.
Instituto de Pesquisa em Engenharia Genética e Biologia Molecular “Dr. Héctor Torres ”. Laboratório de Biologia Molecular da Doença de Chagas. Buenos Aires, Agentina.
Instituto de Pesquisa em Engenharia Genética e Biologia Molecular “Dr. Héctor Torres ”. Laboratório de Biologia Molecular da Doença de Chagas. Buenos Aires, Agentina.
Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil.
Abstract: Laboratory diagnosis of chronic Chagas disease is a troubling factor due to lack of reference tests. The WHO suggests the use of two distinct commercial serological tests in parallel. The performance of commercial immunoassays might fluctuate depending on the antigenic matrices and the local strains of T. cruzi in different geographical settings. The use of antigenic matrices based on chimeric proteins can solve these limitations. Here, we evaluated the diagnostic performance of two chimeric T. cruzi antigens (IBMP-8.1 and -8.4) to diagnose chronic Chagas disease in individuals from endemic South American countries. The IBMP-8.1 and IBMP-8.4 chimeric antigens were expressed as soluble proteins in E. coli and purified using chromatography methods. Reactivity of IBMP-8.1 and IBMP-8.4 was assessed using an in-house ELISA with sera from 122 non-infected and 215 T. cruziinfected individuals from Argentina, Bolivia, and Paraguay. Cut-off values were based on ROC curves and performance parameters were determined using a dichotomous approach. Area under the curve values were > 99.7% for both IBMP-8.1 and IBMP-8.4 antigens. IgG levels in T. cruzi-positive and negative samples were higher for IBMP-8.4 than IBMP-8.1. Both IBMP-8.1 and -8.4 were 100% specific, while IBMP-8.4 were 100% sensitive compared to IBMP-8.1 (95.3%). Admitting RI values of 1.0 ± 0.10 as the inconclusive interval, 6.2% of the samples tested using IBMP-8.1 and 2.1% using IBMP-8.4 fell inside the grey zone. Based on accuracy and diagnostic odds ratio values, IBMP-8.4 presented the best performance. Differences in sensitivity and IgG levels among the samples from Argentina, Bolivia, and Paraguay were not significant. As conclusions, the significance of our findings showed a notable performance of IBMP-8.1 and -8.4 chimeric antigens in diagnosing chronic Chagas disease in individuals from endemic South American countries, confirming our hypothesis that these antigens could be used in geographical areas where distinct T. cruzi DTUs occur.
Keywords: Chagas Disease
Diagnosis
Antigens
Antigen-Antibody Reactions
Keywords in spanish: Enfermedad de Chagas
Reacciones Antígeno-Anticuerpo
keywords: Trypanosoma cruzi
DeCS: Doença de Chagas
Diagnóstico
Antígenos
Reações Antígeno-Anticorpo
Issue Date: 2019
Publisher: Public Library of Science
Citation: DEL-REI, Rodrigo Pimenta et al. Detection of anti-Trypanosoma cruzi antibodies by chimeric antigens in chronic Chagas disease-individuals from endemic South American countries. PLoS ONE, v. 14, n. 4, p. 1–12, 2019.
DOI: 10.1371/journal.pone.0215623
ISSN: 1932-6203
Copyright: open access
Appears in Collections:BA - IGM - Artigos de Periódicos
PR - ICC - Artigos de Periódicos

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