The aim of this study was to identify the species Anisakidae, of interest to public health, parasitizing the cusk-eel, Genypterus brasiliensis Regan, 1903 purchased in markets of Niterói and Rio de Janeiro counties, Brazil, emphasizing their parasite indexes, sites of infection and higienicsanitary importance. Seventy-four specimens of G. brasiliensis were necropsied from October 2002 to September 2003. Twenty fish specimens (27%) were parasitized by live larvae of Anisakidae. The collected species were represented by Anisakis physeteris, Anisakis simplex, Anisakis sp., Pseudoterranova decipiens, Pseudoterranova sp., Hysterothylacium sp., Raphidascaris sp., Contracaecum sp. and Terranova sp. The species Anisakis sp. and A. simplex presented the higher prevalences, both with 13,5% whereas A. physeteris presented the lower prevalence 1,35%. The other species, Pseudoterranova sp., P. decipiens, Hysterothylacium sp., Raphidascaris sp., Contracaecum sp. and Terranova sp. presented prevalences of 10,8%, 5,4%, 2,7%, 4,1%, 1,4% and 1,4%, respectively. The sites of infection were, the mesentery, stomach and intestinal seroses, intestine, ovary and musculature.