Dengue is the most important viral infection transmitted among humans by arthropod-borne. There arecurrently no vaccines or specific therapeutical treatment. Therefore, immunomodulatory compoundsfrom plants have been widely examined for their antiviral effects. Cissampelos sympodialis Eichler, Menis-permaceae, has scientifically proven to present immunomodulatory activities. Here we assessed theantiviral activity of leaf hydroalcoholic extract, warifteine or methylwarifteine from C. sympodialis inan in vitro dengue virus infection model. The results demonstrated that leaf hydroalcoholic extract orwarifteine/methylwarifteine treatment did not reduce dengue virus-Ag+ hepatocyte (Huh-7 cell) ratesin present experimental conditions. However, we assessed the potential antiviral effect of leaf hydroalco-holic extract or warifteine/methylwarifteine on dengue virus-infection by the production of inflammatorymolecules, TNF- , MIF, IL-8 and PGE2.Dengue virus infection enhanced TNF- , MIF, IL-8 and PGE2pro-duction in infected Huh-7 cells and leaf hydroalcoholic extract but not warifteine/methylwarifteinetreatments, significantly reduced these molecules in infected cells. In dengue virus-infected Huh-7 cells,non-structural protein-1 is produced and leaf hydroalcoholic extract significantly inhibited it indepen-dently of alkaloids. Our findings imply that leaf hydroalcoholic extract may attenuate dengue virusinfection in Huh-7 cells by inhibiting the enhanced of pro-inflammatory mediators and non-structuralprotein-1 production induce by dengue virus independently of warifteine/methywarifteine its majorcompound.