Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/3679
Title: Production of monoclonal antibodies against canine leukocytes.
Authors: Aguiar, Paulo Henrique Palis
Santos, Roberto Robson Borges dos
Lima, Carla Andrade
Gomes, Hilton Rios de Sousa
Larangeira, Daniela Farias
Santos, Patrícia Oliveira Meira
Melo, Stella Maria Barrouin
dosSantos, Washington Luis Conrado
Pontes-de-Carvalho, Lain Carlos
Affilliation: Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil.
Universidade Federal da Bahia. Department of Pathology and Clinics, Veterinary School. Salvador, BA, Brasil.
Foundation for Development of Science. Bahiana School of Medicine. Salvador, BA, Brasil.
Foundation for Development of Science. Bahiana School of Medicine. Salvador, BA, Brasil.
Universidade Federal da Bahia. Department of Pathology and Clinics, Veterinary School. Salvador, BA, Brasil.
Universidade Federal da Bahia. Department of Pathology and Clinics, Veterinary School. Salvador, BA, Brasil.
Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil.
Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil.
Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil.
Abstract: A panel of anti-canine leukocyte monoclonal antibodies (MAbs) was produced by immunizing BALB/c mice with canine peripheral blood mononuclear cells (PBMC), either resting or stimulated with concanavalin A (ConA). Three out of 28 clones—IH1, AB6, and HG6–screened by ELISA and producing antibody with the highest specificity for canine cell immunostaining, were subjected to three subsequent subcloning steps by limiting dilution, and selected for further characterization. These MAbs belonged to IgG1 (HG6 and IH1) and IgG2a (AB6) isotypes. The distribution of cell populations expressing the antigen recognized by the antibodies was identified by indirect immunoflorescence on canine PBMC and on tissue sections of lymph node, spleen, liver and skin. The possible crossreactivity with human PBMC was also examined in immunocytochemistry. One of the antibodies specifically recognized macrophages. The MAbs presented here can be foreseen as possible valuable diagnostic and research tools to study immune functions in dogs.
DeCS: Anticorpos Monoclonais
Concanavalina
Animais
Cães
Hibridomas
Humanos
Imunoistoquímica
Leucócitos
Camundongos Endogâmicos BALB C
Camundongos
Issue Date: 2004
Citation: AGUIAR, P. H. P. et al. Production of monoclonal antibodies against canine leukocytes. Hybrid Hybridomics, v. 23, n. 2, p. 127-32, apr. 2004.
ISSN: 1536-8599
Copyright: open access
Appears in Collections:BA - IGM - Artigos de Periódicos

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