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PRODUCTION OF MONOCLONAL ANTIBODIES AGAINST CANINE LEUKOCYTES.
Concanavalina
Animais
Cães
Hibridomas
Humanos
Imunoistoquímica
Leucócitos
Camundongos Endogâmicos BALB C
Camundongos
Autor
Afiliación
Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil.
Universidade Federal da Bahia. Department of Pathology and Clinics, Veterinary School. Salvador, BA, Brasil.
Foundation for Development of Science. Bahiana School of Medicine. Salvador, BA, Brasil.
Foundation for Development of Science. Bahiana School of Medicine. Salvador, BA, Brasil.
Universidade Federal da Bahia. Department of Pathology and Clinics, Veterinary School. Salvador, BA, Brasil.
Universidade Federal da Bahia. Department of Pathology and Clinics, Veterinary School. Salvador, BA, Brasil.
Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil.
Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil.
Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil.
Universidade Federal da Bahia. Department of Pathology and Clinics, Veterinary School. Salvador, BA, Brasil.
Foundation for Development of Science. Bahiana School of Medicine. Salvador, BA, Brasil.
Foundation for Development of Science. Bahiana School of Medicine. Salvador, BA, Brasil.
Universidade Federal da Bahia. Department of Pathology and Clinics, Veterinary School. Salvador, BA, Brasil.
Universidade Federal da Bahia. Department of Pathology and Clinics, Veterinary School. Salvador, BA, Brasil.
Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil.
Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil.
Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil.
Resumen en ingles
A panel of anti-canine leukocyte monoclonal antibodies (MAbs) was produced by immunizing BALB/c mice
with canine peripheral blood mononuclear cells (PBMC), either resting or stimulated with concanavalin A
(ConA). Three out of 28 clones—IH1, AB6, and HG6–screened by ELISA and producing antibody with the
highest specificity for canine cell immunostaining, were subjected to three subsequent subcloning steps by limiting
dilution, and selected for further characterization. These MAbs belonged to IgG1 (HG6 and IH1) and
IgG2a (AB6) isotypes. The distribution of cell populations expressing the antigen recognized by the antibodies
was identified by indirect immunoflorescence on canine PBMC and on tissue sections of lymph node, spleen,
liver and skin. The possible crossreactivity with human PBMC was also examined in immunocytochemistry.
One of the antibodies specifically recognized macrophages. The MAbs presented here can be foreseen as possible
valuable diagnostic and research tools to study immune functions in dogs.
DeCS
Anticorpos MonoclonaisConcanavalina
Animais
Cães
Hibridomas
Humanos
Imunoistoquímica
Leucócitos
Camundongos Endogâmicos BALB C
Camundongos
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