Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/36943
Title: A case of sporotrichosis caused by different Sporothrix brasiliensis strains: mycological, molecular, and virulence analyses
Authors: Oliveira, Manoel Marques E.
Almeida-Paes, Rodrigo
Corrêa-Moreira, Danielly
Borba, Cintia de Moraes
Menezes, Rodrigo Caldas
Freitas, Dayvison Francis Saraiva
Valle, Antonio Carlos Francesconi do
Schubach, Armando de Oliveira
Barros, Monica Bastos de Lima
Nosanchuk, Joshua D.
Gutierrez-Galhardo, Maria Clara
Zancopé-Oliveira, Rosely Maria
Affilliation: Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Laboratório de Pesquisa Clínica em Dermatozoonoses em Animais Domésticos. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Laboratório de Micologia. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Laboratório de Pesquisa Clínica em Dermatozoonoses em Animais Domésticos, Rio de Janeiro, RJ, Brasil / Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Taxonomia, Bioquímica e Bioprospecção de Fungos. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Taxonomia, Bioquímica e Bioprospecção de Fungos. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Laboratório de Pesquisa Clínica em Dermatozoonoses em Animais Domésticos. Rio de Janeiro, RJ, Brasi.
Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Laboratório de Vigilância em Leishmaniose. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Escola Nacional de Saúde Pública. Rio de Janeiro, RJ, Brasil.
Albert Einstein College of Medicine. New York, NY, USA.
Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Laboratório de Micologia. Rio de Janeiro, RJ, Brasil.
Abstract: BACKGROUND Sporotrichosis is a subcutaneous mycosis caused by dimorphic pathogenic fungi belonging to the Sporothrix genus. Pathogenic Sporothrix species typically produce melanin, which is known to be a virulence factor. OBJECTIVES The aim of this study was to perform phenotypic, genotypic, and virulence analyses of two distinct Sporothrix brasiliensis strains isolated from the same lesion on a patient from Rio de Janeiro. METHODS AND FINDINGS Genotypic analyses by partial sequencing of the calmodulin, β-tubulin, and chitin synthase genes, as well as polymerase chain reaction (PCR)-fingerprinting by T3B, M13, and GACA, showed that the isolates were very similar but not identical. Both isolates had similar phenotypic characteristics and effectively produced melanin in their yeast forms, accounting for their ability of causing disease in a murine sporotrichosis model. Remarkably, isolate B was albino in its environmental form but caused more severe disease than the pigmented A isolate. CONCLUSIONS These findings indicate that the patient was infected by two genetically and biologically distinct S. brasiliensis that vary in their production of melanin in their environmental forms. The results underscore the importance of characterizing phenotypically different isolates found in the same clinical specimen or patient.
Keywords: Sporothrix
Genotypic analyses
PCR fingerprint
Experimental murine model
Virulence
Issue Date: 2019
Publisher: Instituto Oswaldo Cruz, Ministério da Saúde
Citation: OLIVEIRA, Manoel Marques E. et al. A case of sporotrichosis caused by different Sporothrix brasiliensis strains: mycological, molecular, and virulence analyses. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 144, p. 1-13, 2019.
DOI: 10.1590/0074-02760190260
ISSN: 0074-0276
Copyright: open access
Appears in Collections:ENSP - Artigos de Periódicos
IOC - Artigos de Periódicos
INI - Artigos de Periódicos

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