Author | Cortez, Erika | |
Author | Stumbo, Ana Carolina | |
Author | Carvalho, Técia Maria U, de | |
Author | Barbosa, Helene Santos | |
Author | Carvalho, Laís | |
Access date | 2019-12-07T13:37:27Z | |
Available date | 2019-12-07T13:37:27Z | |
Document date | 2005 | |
Citation | CORTEZ, Erika et al. NAD(P) H-Oxidase presence in Toxoplasma Gondii Tachyzoite Vacuole during Interaction with IFN-Gamma_actiated human endothelial cells. Journal of Parasitology, v. 91, n. 5, p. 1053-1057, 2005. | pt_BR |
ISSN | 0022-3395 | pt_BR |
URI | https://www.arca.fiocruz.br/handle/icict/37695 | |
Language | eng | pt_BR |
Publisher | American Society of Parasitologists | pt_BR |
Rights | restricted access | pt_BR |
Subject in Portuguese | Toxoplasma gondii | pt_BR |
Subject in Portuguese | Células endotélias de veias umbilicais humanas | pt_BR |
Subject in Portuguese | NAD(P)H-oxidase | pt_BR |
Title | NAD(P)H-oxidase presence in Toxoplasma gondii tachyzoite vacuole during interaction with IFN-gamma-activated human endothelial cells | pt_BR |
Type | Article | pt_BR |
DOI | 10.1645/GE-467R1.1 | |
Abstract | Toxoplasma gondii invades and proliferates in human umbilical vein endothelial cells (HUVEC) where it resides in a parasitophorous vacuole (PV) preventing lysosomal fusion. To study the intracellular outcome of PV containing tachyzoites of T. gondii during interaction with IFN-gamma-activated HUVEC, a quantitative analysis of the T. gondii infection and multiplication was assayed. The quantification of PVs' fusion with lysosomes, ultrastructural examination of phagosome-lysosome fusion, and the localization of NAD(P)H-oxidase activity were also investigated. HUVEC activated with IFN-gamma inhibited T. gondii infection and multiplication by 67.5% and 91.0%, respectively. After 4 hr of infection, 10.2% of IFN-gamma-activated HUVEC exhibited phagosome-lysosome fusion assayed by fluorescence microscopy, which was also observed at the ultrastructural level. Furthermore, the enzyme NAD(P)H-oxidase present at the plasma membrane of activated HUVEC was internalized together with the parasite in 38.0% of the cells. In addition, colocalization of colloidal gold particles and reaction product of NAD(P)H-oxidase in the PV of some activated HUVEC was observed. These results suggest that NAD(P)H-oxidase may participate in a mechanism by which IFN-gamma-activated HUVEC inhibit T. gondii multiplication. | pt_BR |
Affilliation | Universidade do Estado do Rio de Janeiro. IBRAG. Departamento de Histologia e Embriologia. Laboratório de Cultura de Células. Rio de Janeiro, RJ, Brasil. | pt_BR |
Affilliation | Universidade do Estado do Rio de Janeiro. IBRAG. Departamento de Histologia e Embriologia. Laboratório de Cultura de Células. Rio de Janeiro, RJ, Brasil. | pt_BR |
Affilliation | Universidade do Estado do Rio de Janeiro. IBRAG. Departamento de Histologia e Embriologia. Laboratório de Cultura de Células. Rio de Janeiro, RJ, Brasil / Universidade Federal do Rio de Janeiro. Laboratório de Ultraestrutura Celular H Meyer. Rio de Janeiro, RJ, Brasil. | pt_BR |
Affilliation | Universidade do Estado do Rio de Janeiro. IBRAG. Departamento de Histologia e Embriologia. Laboratório de Cultura de Células. Rio de Janeiro, RJ, Brasil / Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Estrutural. Rio de Janeiro, RJ, Brasil. | pt_BR |
Affilliation | Universidade do Estado do Rio de Janeiro. IBRAG. Departamento de Histologia e Embriologia. Laboratório de Cultura de Células. Rio de Janeiro, RJ, Brasil. | pt_BR |
Subject | Toxoplasma gondii | pt_BR |
Subject | NAD(P)H-oxidase | pt_BR |
Subject | IFN-g–activated HUVEC | pt_BR |
Subject | Human endothelial cells | pt_BR |
e-ISSN | 1937-2345 | |
Embargo date | 2025-01-01 | |