Por favor, use este identificador para citar o enlazar este ítem:
https://www.arca.fiocruz.br/handle/icict/37737
Tipo
ArtículoDerechos de autor
Acceso abierto
Fecha del embargo
2020-12-10
Colecciones
- INI - Artigos de Periódicos [3498]
Metadatos
Mostrar el registro completo del ítem
GENOTYPE AND MATING TYPE DISTRIBUTION WITHIN CLINICAL CRYPTOCOCCUS NEOFORMANS AND CRYPTOCOCCUS GATTII ISOLATES FROM PATIENTS WITH CRYPTOCOCCAL MENINGITIS IN UBERABA, MINAS GERAIS, BRAZIL. 2009
Cryptococcus gatti
Mating type
URA5-RFLP
PCR-fingerprinting
Autor
Afiliación
Universidade Federal do Triângulo Mineiro. Uberaba, MG, Brasil.
Universidade Federal do Triângulo Mineiro. Uberaba, MG, Brasil.
Universidade Federal do Triângulo Mineiro. Uberaba, MG, Brasil.
Universidade de São Paulo. Escola de Medicina de Ribeirão Preto. Departamento de Biologia Celular e Molecular. Ribeirão Preto, SP, Brasil.
Fundação Oswaldo Cruz. Instituto de Pesquisa Clínica Evandro Chagas. Laboratório de Micologia. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto de Pesquisa Clínica Evandro Chagas. Laboratório de Micologia. Rio de Janeiro, RJ, Brasil.
Universidade Federal do Triângulo Mineiro. Uberaba, MG, Brasil.
Universidade Federal do Triângulo Mineiro. Uberaba, MG, Brasil.
Universidade Federal do Triângulo Mineiro. Uberaba, MG, Brasil.
Universidade de São Paulo. Escola de Medicina de Ribeirão Preto. Departamento de Biologia Celular e Molecular. Ribeirão Preto, SP, Brasil.
Fundação Oswaldo Cruz. Instituto de Pesquisa Clínica Evandro Chagas. Laboratório de Micologia. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto de Pesquisa Clínica Evandro Chagas. Laboratório de Micologia. Rio de Janeiro, RJ, Brasil.
Universidade Federal do Triângulo Mineiro. Uberaba, MG, Brasil.
Resumen en ingles
We molecularly characterized 81 cryptococcal isolates recovered from cerebrospinal fluid samples of 77 patients diagnosed between 1998 and 2007 as having cryptococcal meningitis in Uberaba Minas Gerais, Brazil. Fifty-seven (74%) were male with a mean age 35.6 years. Seventy-two (88.9%) of the isolates were from 68 AIDS patients and cryptococcosis was the fi rst AIDS-defi ning condition in 38 (55.9%) patients. Cryptococcosis
and AIDS were simultaneously diagnosed in 25 (65.8%) of these 38 patients. Genotypes were characterized through the use of URA5 restriction fragment length polymorphisms analysis, the genetic variability was determined using PCR-fi ngerprinting with the minisatellite-specifi c primer M13, and the mating type and serotypes were established by PCR. Seventy-six of the 81 isolates were Cryptococcus neoformans (93.8%), while the remaining fi ve were C. gattii (6.1%), but all were mating type α. C. neoformans isolates were genotype VNI (serotype A), while C. gattii isolates were VGII. Four of the latter isolates were identical, but only two were from AIDS patients. Six of the nine isolates from non-AIDS patients were VNI. PCR fi ngerprints of the isolates from two of the three AIDS patients with clinical relapse were 100% identical. The predominance of VNI and mating type α is in accordance with data from other parts of the world. The occurrence of VGII in Minas Gerais indicates a geographical expansion within Brazil.
Palabras clave en ingles
Cryptococcus neoformansCryptococcus gatti
Mating type
URA5-RFLP
PCR-fingerprinting
Compartir