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dc.contributor.authorCaulfield, John P.
dc.contributor.authorLenzi, Henrique Leonel
dc.contributor.authorElsas, Pedro Paulo Xavier
dc.contributor.authorDessein, Alain J.
dc.identifier.citationCAULFIELD, J. P. et al. Ultrastructure of the attack of eosinophils stimulated by blood mononuclear cell products on schistosomula of Schistosoma mansoni. American Journal of Pathology, New York, v. 120, n.3, p. 380-90, Sep. 1985.
dc.descriptionAcknowledgments The authors thank Catherine Cianci for her excellent work in preparing and evaluating the electron micrographs. We also thank Ann Hein, Jennifer Hus, and Jack Quinn for their excellent technical assistance and Dee Condon, Carolyn McDowell, and Laurie Walker for excellent secretarial assistance.
dc.rightsopen access
dc.titleUltrastructure of the attack of eosinophils stimulated by blood mononuclear cell products on schistosomula of Schistosoma mansoni.
dc.description.abstractenPurified human eosinophils were treated with peripheral blood mononuclear cell supernatants containing eosinophil cytotoxic enhancing activity (ECEA). Schistosomula of Schistosoma mansoni which had been coated either with antibody (Ab) from the sera of infected patients or with the lectin concanavalin A (Con A) were incubated with ECEA-treated and untreated cells for 2 minutes to 12 hours and examined ultrastructurally. Killing was assayed at 18 hours. ECEA caused an increase in the killing of Ab-coated worms, but Con-A-coated worms were not killed by either ECEA-treated or untreated cells. Eosinophils began to degranulate on Ab-coated worms within 2 minutes and continued to degranulate, so that by 12 hours about half of the parasites had greater than 50% of their surface covered by discharge material. The ECEA-treated cells degranulated more than the untreated cells. There was much less discharge material on Con-A-coated worms than on Ab-coated worms. Eosinophils adhered to discharge material on the surface of both Ab- and Con-A-coated parasites. At 3 and 12 hours, lysed cells and cell fragments were also seen adhering to discharge material. In the absence of discharge material the cells adhered to residual glycocalyx or to the tegumental outer membrane. These studies suggest that eosinophils kill schistosomula by progressively degranulating onto their surface over many hours and that the increased toxicity caused by ECEA is due to an increase in discharge.
dc.creator.affilliationHarvard Medical School. Departments of Pathology and Medicine, Boston, MA, Estados Unidos / Brigham and Women's Hospital. Division of Rheumatology and Immunology. Boston, MA, Estados Unidos
dc.creator.affilliationFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Patologia. Rio de Janeiro, RJ, Brasil
dc.creator.affilliationINSERM CNRS. Centre d'Immunologiede Marseille-Luminy, Marseille França
dc.creator.affilliationNSERM CNRS. Centre d'Immunologiede Marseille-Luminy, Marseille França
dc.subject.enSchistosoma mansoni - ultrastructure
dc.subject.enEosinophils - ultrastructure
dc.subject.enSchistosomula of Schistosoma mansoni
dc.subject.decsSchistosoma mansoni - ultraestrutura
dc.subject.decsEosinophils - ultraestrutura
Appears in Collections:IOC - Artigos de Periódicos
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