Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/3798
Title: Lutzomyia longipalpis salivary gland homogenate impairs cytokine production and costimulatory molecule expression on human monocytes and dendritic cells
Authors: Costa, Dirceu Joaquim
Favali, Cecilia Beatriz Fiuza
Clarêncio, Jorge
Afonso, Lilian Maria
Conceição, Viviane
Miranda, José Carlos
Titus, Richard G.
Valenzuela, Jesus G.
Barral Netto, Manoel
Barral, Aldina Maria Prado
Brodskyn, Claudia Ida
Affilliation: Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil.
Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil.
Universidade Federal da Bahia. Instituto de Ciências da Saúde. Salvador, BA, Brasil.
Universidade Federal da Bahia. Instituto de Ciências da Saúde. Salvador, BA, Brasil.
Universidade Federal da Bahia. Instituto de Ciências da Saúde. Salvador, BA, Brasil.
Universidade Federal da Bahia. Instituto de Ciências da Saúde. Salvador, BA, Brasil.
Colorado State University. College of Veterinary Medicine and Biomedical Sciences. Colorado, USA.
National Institute of Health. Bethesda, Maryland, USA.
Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil.
Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil.
Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil.
Abstract: In this report, we describe an investigation of the effects of Lutzomyia longipalpis sand fly salivary gland homogenates (SGH) on cytokine production and expression of costimulatory molecules on human monocytes, macrophages (Mphis), and dendritic cells (DCs). SGH of L. longipalpis induced an increase in interleukin-6 (IL-6), IL-8 and IL-12p40 production but a decrease in tumor necrosis factor alpha and IL-10 production by lipopolysaccharida (LPS)-stimulated monocytes. We also examined the expression of costimulatory molecules on the surface of monocytes, Mphis, and DCs. Whereas SGH affected the expression of these molecules on monocytes and Mphis, it had little effect on these molecules on DCs. However, when DCs were generated from human monocytes in the presence of SGH, SGH inhibited the expression of costimulatory molecules. In addition, a decrease in the maturation of DCs induced by CD40L was observed in the presence of SGH. Finally, preincubating SGH with human sera containing anti-SGH-specific antibodies abolished the effects of SGH on cytokine production by LPS-stimulated monocytes.
DeCS: Citocinas
Psychodidae
Glândulas Salivares
Diferenciação Celular
Células Dendríticas
Insetos Vetores
Leishmania
Leishmaniose
Lipopolissacarídeos
Macrófagos
Monócitos
Testes de Neutralização
Issue Date: 2004
Citation: COSTA, Dirceu Joaquim et al. Lutzomyia longipalpis salivary gland homogenate impairs cytokine production and costimulatory molecule expression on human monocytes and dendritic cells. InfectIon and Immunity, v. 72, n. 3, p. 1298-1305, Mar. 2004.
DOI: 10.1128/IAI.72.3.1298–1305.2004
ISSN: 0019-9567
Copyright: open access
Appears in Collections:BA - IGM - Artigos de Periódicos

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