Author | Freitas, Mônica S. | |
Author | Da Poian, Andrea T. | |
Author | Barth, Ortrud M. | |
Author | Rebello, Moacyr A. | |
Author | Silva, Jerson L. | |
Author | Gaspar, Luciane P. | |
Access date | 2020-01-21T10:48:19Z | |
Available date | 2020-01-21T10:48:19Z | |
Document date | 2006 | |
Citation | FREITAS, Mônica S. et al. The fusogenic state of Mayaro virus induced by low pH and by hydrostatic pressure. Cell Biochemistry and Biophysics, v. 44, p. 325-335, Mar. 2006. | pt_BR |
ISSN | 1085-9195 | pt_BR |
URI | https://www.arca.fiocruz.br/handle/icict/39329 | |
Language | eng | pt_BR |
Publisher | Springer | pt_BR |
Rights | restricted access | pt_BR |
Subject in Portuguese | Vírus Mayaro | pt_BR |
Subject in Portuguese | Estado fusogênico | pt_BR |
Subject in Portuguese | Pressão hidrostática | pt_BR |
Subject in Portuguese | pH baixo | pt_BR |
Subject in Portuguese | Inativação | pt_BR |
Title | The fusogenic state of Mayaro virus induced by low pH and by hydrostatic pressure | en |
Type | Article | pt_BR |
DOI | 10.1385/CBB:44:3:325 | pt_BR |
Abstract | Mayaro virus is an enveloped virus that belongs to the Alphavirus genus. To gain insight into the mechanism involved in Mayaro virus membrane fusion, we used hydrostatic pressure and low pH to isolate a fusion-active state of Mayaro glycoproteins. In response to pressure, E1 glycoprotein undergoes structural changes resulting in the formation of a stable conformation. This state was characterized and correlated to that induced by low pH as measured by intrinsic fluorescence, 4,4′-dianilino-1,1′-binaphthyl-5,5′-disulfonic acid, dipotassium salt fluorescence, fluorescence resonance energy transfer, electron microscopy, and sodium dodecyl sulfate-polyacrylamide gel electrophoresis. In parallel, we used a neutralization assay to show that Mayaro virus in the fusogenic state retained most of the original immunogenic properties and could elicit high titers of neutralizing antibodies. | en |
Affilliation | Universidade Federal do Rio de Janeiro. Centro Nacional de Ressonância Magnética Nuclear de Macromoléculas Jiri Jonas. Instituto de Bioquímica Médica. Programa de Biologia Estrutural. Rio de Janeiro, RJ, Brasil. | pt_BR |
Affilliation | Universidade Federal do Rio de Janeiro. Centro Nacional de Ressonância Magnética Nuclear de Macromoléculas Jiri Jonas. Instituto de Bioquímica Médica. Programa de Biologia Estrutural. Rio de Janeiro, RJ, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Virologia. Rio de Janeiro, RJ, Brasil. | pt_BR |
Affilliation | Universidade Federal do Rio de Janeiro. Instituto de Microbiologia Prof. Paulo de Góes. Departamento de Virologia. Rio de Janeiro, RJ, Brasil. | pt_BR |
Affilliation | Universidade Federal do Rio de Janeiro. Centro Nacional de Ressonância Magnética Nuclear de Macromoléculas Jiri Jonas. Instituto de Bioquímica Médica. Programa de Biologia Estrutural. Rio de Janeiro, RJ, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto de Tecnologia em Imunobiológicos. Vice Diretoria de Desenvolvimento Tecnológico. Rio de Janeiro, RJ, Brasil. | pt_BR |
Subject | Mayaro virus | pt_BR |
Subject | Fusogenic state | pt_BR |
Subject | Hydrostatic pressure | pt_BR |
Subject | Low pH | pt_BR |
Subject | Inactivation | pt_BR |
e-ISSN | 1559-0283 | pt_BR |
Embargo date | 2022-01-01 | |