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EXPLORING THE "LATIN AMERICAN MEDITERRANEAN" FAMILY AND THE RDRIO LINEAGE IN MYCOBACTERIUM TUBERCULOSIS ISOLATES FROM PARAGUAY, ARGENTINA AND VENEZUELA
Díaz Acosta, Chyntia Carolina et al. | Date Issued: 2019
Author
Díaz Acosta, Chyntia Carolina
Russomando, Graciela
Candia, Norma
Ritacco, Viviana
Vasconcellos, Sidra E. G.
Moreira, Marcia de Berrêdo Pinho
Romero, Nilda J, de
Morcillo, Nora
De Waard, Jacobus Henri
Gomes, Harrison Magdinier
Suffys, Philip Noel
Affilliation
Universidad Nacional de Asunción. Instituto de Investigaciones en Ciencias de la Salud. Departamento de Biología Molecular y Biotecnología. Asunción, Paraguay / Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Molecular aplicada às Micobactérias. Rio de Janeiro, RJ, Brasil.
Universidad Nacional de Asunción. Instituto de Investigaciones en Ciencias de la Salud. Departamento de Biología Molecular y Biotecnología. Asunción, Paraguay.
Universidad Nacional de Asunción. Instituto de Investigaciones en Ciencias de la Salud. Departamento de Biología Molecular y Biotecnología. Asunción, Paraguay.
Instituto Nacional de Enfermedades Infecciosas, ANLIS “Carlos G. Malbran”. Servicio de Micobacterias. Buenos Aires. Argentina.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Molecular aplicada às Micobactérias. Rio de Janeiro, RJ, Brasil..
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Microbiologia Celular, Rio de Janeiro, RJ, Brasil.
Laboratorio Central de Salud Pública, MSP y BS. Asunción, Paraguay.
Instituto Nacional de Enfermedades Respiratorias Emilio Coni. Buenos Aires, Argentina.
Instituto de Biomedicina. Laboratorio de Tuberculosis. Caracas, Venezuela / Universidad de Las Américas Facultad de Ciencias de la Salud. One Health Research Group. Quito, Ecuador.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Molecular aplicada às Micobactérias. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Molecular aplicada às Micobactérias. Rio de Janeiro, RJ, Brasil.
Abstract
The Latin American & Mediterranean (LAM) spoligotype family is one of the most successful genotype of Mycobacterium tuberculosis worldwide and particularly prevalent in South-America. Within this family, a sublineage named Region of Difference Rio (RDRio) was reported initially in Brazil and is characterized by a genomic deletion of about 26.3 kb. This lineage seems to show a specific adaptation to the Euro-Latin American population. In this context, we sought to evaluate the LAM family and the presence of the RDRio genotype in samples from three Latin American countries including Paraguay, Venezuela and Argentina. To detect LAM strains reliably we applied a typing scheme using spoligotyping, 12 loci MIRU-VNTR, the Ag85C103 SNP and the regions of difference RDRio and RD174. IS6110-RFLP results were also used when available.
Keywords in Portuguese
Mycobacterium tuberculosis
Genotipagem
Paraguai
América do Sul
Família de spoligótipos
 
Keywords
Mycobacterium tuberculosis
LAM
Genotyping
Paraguay
South-America
 
Publisher
BMC
Citation
COSTA, Cynthia Carolina Díaz et al. Exploring the “Latin American Mediterranean” family and the RDRio lineage in Mycobacterium tuberculosis isolates from Paraguay, Argentina and Venezuela; BMC Microbiology, v. 19, n. 131, p. 1-13, 2019.
DOI
10.1186/s12866-019-1479-6
ISSN
1471-2180