Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/42004
Title: Reporter Replicons for Antiviral Drug Discovery against Positive Single-Stranded RNA Viruses
Authors: Fernandes, Rafaela S.
Freire, Marjorie C. L. C.
Bueno, Renata V.
Godoy, Andre S.
Gil, Laura H. V. G.
Oliva, Glaucius
Affilliation: Universidade de São Paulo. Instituto de Física de São Carlos. São Carlos, SP, Brasil.
Universidade de São Paulo. Instituto de Física de São Carlos. São Carlos, SP, Brasil.
Universidade de São Paulo. Instituto de Física de São Carlos. São Carlos, SP, Brasil.
Universidade de São Paulo. Instituto de Física de São Carlos. São Carlos, SP, Brasil.
Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Recife, PE, Brasil.
Universidade de São Paulo. Instituto de Física de São Carlos. São Carlos, SP, Brasil.
Abstract: Single-stranded positive RNA ((+) ssRNA) viruses include several important human pathogens. Some members are responsible for large outbreaks, such as Zika virus, West Nile virus, SARS-CoV, and SARS-CoV-2, while others are endemic, causing an enormous global health burden. Since vaccines or specific treatments are not available for most viral infections, the discovery of direct-acting antivirals (DAA) is an urgent need. Still, the low-throughput nature of and biosafety concerns related to traditional antiviral assays hinders the discovery of new inhibitors. With the advances of reverse genetics, reporter replicon systems have become an alternative tool for the screening of DAAs. Herein, we review decades of the use of (+) ssRNA viruses replicon systems for the discovery of antiviral agents. We summarize different strategies used to develop those systems, as well as highlight some of the most promising inhibitors identified by the method. Despite the genetic alterations introduced, reporter replicons have been shown to be reliable systems for screening and identification of viral replication inhibitors and, therefore, an important tool for the discovery of new DAAs.
Keywords: Replicon Systems
(+) ssRNA viruses
Direct-acting antivirals
Drug Discovery
SARS-CoV-2
COVID-19
Coronavirus
Issue Date: 2020
Publisher: MDPI
Citation: FERNANDES, Rafaela S. et al. Reporter Replicons for Antiviral Drug Discovery against Positive Single-Stranded RNA Viruses. Viruses, v .12, n 598, p. 2-22, 2020.
DOI: 10.3390/v12060598
ISSN: 1999-4915
Copyright: open access
Appears in Collections:PE - IAM - Artigos de Periódicos
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