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https://www.arca.fiocruz.br/handle/icict/43400
GENETIC ENGINEERING AS A STRATEGY TO IMPROVE THE THERAPEUTIC EFFICACY OF MESENCHYMAL STEM/STROMAL CELLS IN REGENERATIVE MEDICINE
Engenharia genética
Medicina regenerativa
Terapia celular
Terapia de genes
Células estromais
Genetic engineering
Regenerative medicine
Cell therapy
Gene therapy
Stromal cells
Author
Affilliation
Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil / Health Institute of Technology. Salvador, BA, Brazil.
Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil.
Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil.
Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil / Health Institute of Technology. Salvador, BA, Brazil.
Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil / Health Institute of Technology. Salvador, BA, Brazil.
Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil.
University of Modena and Reggio Emilia. Laboratory of Cellular Therapy. Division of Oncology. Modena, Italy.
University of Modena and Reggio Emilia. Laboratory of Cellular Therapy. Division of Oncology. Modena, Italy.
University of Modena and Reggio Emilia. Department of Medical and Surgical Sciences for Children & Adults. Division of Plastic Surgery. Modena, Italy.
Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil / Health Institute of Technology. Salvador, BA, Brazil / National Institute of Science and Technology for Regenerative Medicine. Rio de Janeiro, RJ, Brazil.
Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil / Health Institute of Technology. Salvador, BA, Brazil / National Institute of Science and Technology for Regenerative Medicine. Rio de Janeiro, RJ, Brazil.
Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil.
Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil.
Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil / Health Institute of Technology. Salvador, BA, Brazil.
Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil / Health Institute of Technology. Salvador, BA, Brazil.
Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil.
University of Modena and Reggio Emilia. Laboratory of Cellular Therapy. Division of Oncology. Modena, Italy.
University of Modena and Reggio Emilia. Laboratory of Cellular Therapy. Division of Oncology. Modena, Italy.
University of Modena and Reggio Emilia. Department of Medical and Surgical Sciences for Children & Adults. Division of Plastic Surgery. Modena, Italy.
Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil / Health Institute of Technology. Salvador, BA, Brazil / National Institute of Science and Technology for Regenerative Medicine. Rio de Janeiro, RJ, Brazil.
Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil / Health Institute of Technology. Salvador, BA, Brazil / National Institute of Science and Technology for Regenerative Medicine. Rio de Janeiro, RJ, Brazil.
Abstract
Mesenchymal stem/stromal cells (MSCs) have been widely studied in the field of regenerative medicine for applications in the treatment of several disease settings. The therapeutic potential of MSCs has been evaluated in studies in vitro and in vivo, especially based on their anti-inflammatory and pro-regenerative action, through the secretion of soluble mediators. In many cases, however, insufficient engraftment and limited beneficial effects of MSCs indicate the need of approaches to enhance their survival, migration and therapeutic potential. Genetic engineering emerges as a means to induce the expression of different proteins and soluble factors with a wide range of applications, such as growth factors, cytokines, chemokines, transcription factors, enzymes and microRNAs. Distinct strategies have been applied to induce genetic modifications with the goal to enhance the potential of MCSs. This review aims to contribute to the update of the different genetically engineered tools employed for MSCs modification, as well as the factors investigated in different fields in which genetically engineered MSCs have been tested.
Keywords in Portuguese
Células-tronco mesenquimaisEngenharia genética
Medicina regenerativa
Terapia celular
Terapia de genes
Células estromais
Keywords
Mesenchymal stemGenetic engineering
Regenerative medicine
Cell therapy
Gene therapy
Stromal cells
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