| Author | Rocha, Vinícius Pinto Costa | |
| Author | Dacher, Mariko | |
| Author | Young, Simon Alan | |
| Author | Kolokousi, Foteini | |
| Author | Efstathiou, Antonia | |
| Author | Späth, Gerald Frank | |
| Author | Soares, Milena Botelho Pereira | |
| Author | Smirlis, Despina | |
| Access date | 2020-09-22T16:46:25Z | |
| Available date | 2020-09-22T16:46:25Z | |
| Document date | 2020 | |
| Citation | ROCHA, Vinícius Pinto et al. CostaLeishmania dual-specificity tyrosine-regulated kinase 1 (DYRK1) is required for sustaining Leishmania stationary phase phenotype. Molecular Microbiology, p. 1-21, 2020. | pt_BR |
| ISSN | 0950-382X | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/43539 | |
| Sponsorship | International Division of the Institute
Pasteur, Grant/Award Number: ACIP/
A13-2013; FAPESB/CAPES, Grant/Award
Number: # PET0042/2013; Financiadora de
Estudos e Projetos, Grant/Award Number:
02.13.0137.00; European Union and the
National Ministry of Education and Religion
Affairs under the Operational Strategic
Reference Framework (NSRF 2007-2013)
and the General Secretariat of Research and
Technology (GSRT), Grant/Award Number:
Action “KRIPIS I” (MIS 450598 | pt_BR |
| Language | eng | pt_BR |
| Publisher | Wiley | pt_BR |
| Rights | restricted access | pt_BR |
| Subject in Portuguese | DYRK1 | pt_BR |
| Subject in Portuguese | Crescimento | pt_BR |
| Subject in Portuguese | Quinase | pt_BR |
| Subject in Portuguese | Leishmania | pt_BR |
| Subject in Portuguese | Estacionário | pt_BR |
| Title | Leishmania dual-specificity tyrosine-regulated kinase 1 (DYRK1) is required for sustaining Leishmania stationary phase phenotype | pt_BR |
| Type | Article | |
| DOI | 10.1111/mmi.14464 | pt_BR |
| Abstract | Although the multiplicative and growth-arrested states play key roles in Leishmania
development, the regulators of these transitions are largely unknown. In an attempt
to gain a better understanding of these processes, we characterised one member
of a family of protein kinases with dual specificity, LinDYRK1, which acts as a stasis
regulator in other organisms. LinDYRK1 overexpressing parasites displayed a decrease
in proliferation and in cell cycle re-entry of arrested cells. Parasites lacking
LinDYRK1 displayed distinct fitness phenotypes in logarithmic and stationary growth
phases. In logarithmic growth phase, LinDYRK1–/– parasites proliferated better than
control lines, supporting a role of this kinase in stasis, while in stationary growth
phase, LinDYRK1–/– p arasites h ad i mportant d efects a s t hey r ounded u p, a ccumulated
vacuoles and lipid bodies and displayed subtle but consistent differences in
lipid composition. Moreover, they expressed less metacyclic-enriched transcripts,
displayed increased sensitivity to complement lysis and a significant reduction in survival
within peritoneal macrophages. The distinct LinDYRK1–/– g rowth p hase p henotypes
were mirrored by the distinct LinDYRK1 localisations in logarithmic (mainly
in flagellar pocket area and endosomes) and late stationary phase (mitochondrion).
Overall, this work provides first evidence for the role of a DYRK family member in
sustaining promastigote stationary phase phenotype and infectivity. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil / Hospital São Rafael. Centro de Biotecnologia e Terapia Celular. Salvador, BA, Brasil. | pt_BR |
| Affilliation | Institut Pasteur and INSERM. Unité de Parasitologie Moléculaire et Signalisation. Department of Parasites and Insect Vectors. Paris, France. | pt_BR |
| Affilliation | The University of St. Andrews. School of Biology. Biomedical Sciences Research Complex. St. Andrews, UK. | pt_BR |
| Affilliation | Hellenic Pasteur Institute. Molecular Parasitology Laboratory, Microbiology Department. Athens, Greece. | pt_BR |
| Affilliation | Hellenic Pasteur Institute. Molecular Parasitology Laboratory, Microbiology Department. Athens, Greece. | pt_BR |
| Affilliation | Institut Pasteur and INSERM. Unité de Parasitologie Moléculaire et Signalisation. Department of Parasites and Insect Vectors. Paris, France. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil / Hospital São Rafael. Centro de Biotecnologia e Terapia Celular. Salvador, BA, Brasil. | pt_BR |
| Affilliation | Hellenic Pasteur Institute. Molecular Parasitology Laboratory, Microbiology Department. Athens, Greece. | pt_BR |
| Subject | DYRK1 | pt_BR |
| Subject | Growth | pt_BR |
| Subject | Kinase | pt_BR |
| Subject | Leishmania | pt_BR |
| Subject | Stationary | pt_BR |
