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DIAGNOSIS OF DENGUE BY USING REVERSE TRANSCRIPTASE-POLYMERASE CHAIN REACTION
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Abstract
A rapid identification of dengue viruses from clinical samples by using a nested reverse transcriptasepolymerase chain reaction (RT-PCR) procedure was carried out for diagnostic and epidemiological
purposes. RT-PCR identified DEN-1 and DEN-2 viruses in 41% (41/100) of previously confirmed
cases and provided an accurate confirmation of DHF in four fatal cases. RT-PCR was also useful for
detecting and typing dengue viruses in suspected cases, allowing a rapid identification of new serotypes
in endemic areas.
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