Author | Honório, Nildimar Alves | |
Author | Câmara, Daniel Cardoso Portela | |
Author | Wiggins, Keenan Wiggins | |
Author | Eastmond, Bradley | |
Author | Alto, Barry Wilmer | |
Access date | 2021-01-07T14:42:08Z | |
Available date | 2021-01-07T14:42:08Z | |
Document date | 2020 | |
Citation | HONÓRIO, Nildimar Alves et al. High-Throughput Method for Detection of Arbovirus Infection of Saliva in Mosquitoes Aedes aegypti and Ae. albopictus. Viruses, v. 12, 1343, 18p, Nov. 2020. | pt_BR |
ISSN | 1999-4915 | pt_BR |
URI | https://www.arca.fiocruz.br/handle/icict/45527 | |
Language | eng | pt_BR |
Publisher | MDPI | pt_BR |
Rights | open access | pt_BR |
Subject in Portuguese | Mosquito vetores | pt_BR |
Subject in Portuguese | Competência vetorial | pt_BR |
Subject in Portuguese | Ensaio de saliva | pt_BR |
Subject in Portuguese | Transmissão | pt_BR |
Subject in Portuguese | Vigilância de arbovírus | pt_BR |
Title | High-Throughput Method for Detection of Arbovirus Infection of Saliva in Mosquitoes Aedes aegypti and Ae. albopictus | pt_BR |
Type | Article | pt_BR |
DOI | 10.3390/v12111343 | |
Abstract | Vector competence refers to the ability of a vector to acquire, maintain, and transmit a
pathogen. Collecting mosquito saliva in medium-filled capillary tubes has become the standard for
approximating arbovirus transmission. However, this method is time-consuming and labor-intensive.
Here we compare the capillary tube method to an alternative high-throughput detection method the
collection of saliva on paper cards saturated with honey, with (FTA card) and without (filter paper)
reagents for the preservation of nucleic acid for Aedes aegypti and Aedes albopictus mosquitoes infected
with two emerging genotypes of the chikungunya virus (CHIKV). Model results showed that the
Asian genotype CHIKV dissemination in the harvested legs of both Ae. aegypti and Ae. albopictus
increased the odds of females having a positive salivary infection and higher salivary viral titers,
while for the IOL genotype the same e ect was observed only for Ae. aegypti. Of the three tested
detection methods, the FTA card was significantly more e ective at detecting infected saliva of
Ae. aegypti and Ae. albopictus females than the capillary tube and filter paper was as e ective as the
capillary tube for the Asian genotype. We did not find significant e ects of the detection method in
detecting higher viral titer for both Asian and IOL genotypes. Our results are discussed in light of the
limitations of the di erent tested detection methods. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Mosquitos Transmissores de Hematozoários. Rio de Janeiro, RJ, Brasil / Fundação Oswaldo Cruz. Núcleo Operacional Sentinela de Mosquitos Vetores-Nosmove. Rio de Janeiro, RJ, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Mosquitos Transmissores de Hematozoários. Rio de Janeiro, RJ, Brasil / Fundação Oswaldo Cruz. Núcleo Operacional Sentinela de Mosquitos Vetores-Nosmove. Rio de Janeiro, RJ, Brasil. | pt_BR |
Affilliation | IFAS, Department of Entomology and Nematology, Florida Medical Entomology Laboratory. University of Florida. Vero Beach, FL, USA. | pt_BR |
Affilliation | IFAS, Department of Entomology and Nematology, Florida Medical Entomology Laboratory. University of Florida. Vero Beach, FL, USA. | pt_BR |
Affilliation | IFAS, Department of Entomology and Nematology, Florida Medical Entomology Laboratory. University of Florida. Vero Beach, FL, USA. | pt_BR |
Subject | Mosquito vectors | pt_BR |
Subject | Vector competence | pt_BR |
Subject | Saliva assay | pt_BR |
Subject | Transmission | pt_BR |
Subject | Arbovirus surveillance | pt_BR |