Author | Barros, Juliana Helena da Silva | |
Author | Roque, André Luiz Rodrigues | |
Author | Xavier, Samanta Cristina das Chagas | |
Author | Nascimento, Kátia Cristina Silva | |
Author | Toma, Helena Keiko | |
Author | Madeira, Maria de Fatima | |
Access date | 2021-01-26T18:31:04Z | |
Available date | 2021-01-26T18:31:04Z | |
Document date | 2020 | |
Citation | BARROS, Juliana Helena da Silva et al. Biological and Genetic Heterogeneity in Trypanosoma dionisii Isolates from Hematophagous and Insectivorous Bats. Pathogens, v. 9, n. 736, 12p, 2020. | pt_BR |
ISSN | 2076-0817 | pt_BR |
URI | https://www.arca.fiocruz.br/handle/icict/45832 | |
Language | eng | pt_BR |
Publisher | MDPI | pt_BR |
Rights | open access | pt_BR |
Subject in Portuguese | Chiroptera | pt_BR |
Subject in Portuguese | Schizotrypanum | pt_BR |
Subject in Portuguese | Caracterização molecular | pt_BR |
Subject in Portuguese | Metodologias integrativas | pt_BR |
Subject in Portuguese | Caracterização biológica | pt_BR |
Subject in Portuguese | Diferences entre isolados | pt_BR |
Subject in Portuguese | Trypanosomatina | pt_BR |
Subject in Portuguese | Trypanosoma cruzi | pt_BR |
Title | Biological and Genetic Heterogeneity in Trypanosoma dionisii Isolates from Hematophagous and Insectivorous Bats | pt_BR |
Type | Article | pt_BR |
DOI | 10.3390/pathogens9090736 | |
Abstract | This study describes the morphological, biochemical, and molecular di erences among
Trypanosoma dionisii isolates from hemocultures of hematophagous (Desmodus rotundus; n = 2) and
insectivorous (Lonchorhina aurita; n = 1) bats from the Atlantic Rainforest of Rio de Janeiro, Brazil.
Fusiform epimastigotes from the hematophagous isolates were elongated, whereas those of the
insectivorous isolate were stumpy, reflected in statistically evident di erences in the cell body and
flagellum lengths. In the hemocultures, a higher percentage of trypomastigote forms (60%) was
observed in the hematophagous bat isolates than that in the isolate from the insectivorous bat (4%),
which demonstrated globular morphology. Three molecular DNA regions were analyzed: V7V8 (18S
rDNA), glycosomal glyceraldehyde 3-phosphate dehydrogenase gene, and mitochondrial cytochrome
b gene. The samples were also subjected to multilocus enzyme electrophoresis and random amplified
polymorphic DNA analysis. All isolates were identified as T. dionisii by phylogenetic analysis.
These sequences were clustered into two separate subgroups with high bootstrap values according
to the feeding habits of the bats from which the parasites were isolated. However, other T. dionisii
samples from bats with di erent feeding habits were found in the same branch. These results support
the separation of the three isolates into two subgroups, demonstrating that di erent subpopulations
of T. dionisii circulate among bats. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia de Tripanosomatídeos. Rio de Janeiro, RJ, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia de Tripanosomatídeos. Rio de Janeiro, RJ, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia de Tripanosomatídeos. Rio de Janeiro, RJ, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia de Tripanosomatídeos. Rio de Janeiro, RJ, Brasil. | pt_BR |
Affilliation | Universidade Federal do Rio de Janeiro. Faculdade de Farmácia. Laboratório de Diagnóstico Molecular e Hematologia. Rio de Janeiro, RJ, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Laboratório de Vigilância em Leishmanioses. Rio de Janeiro, RJ, Brasil. | pt_BR |
Subject | Chiroptera | pt_BR |
Subject | Trypanosomatidae | pt_BR |
Subject | Schizotrypanum | pt_BR |
Subject | Integrative methodologies | pt_BR |
Subject | Molecular characterization | pt_BR |
Subject | Biological characterization | pt_BR |
Subject | Diferences among isolates | pt_BR |