Please use this identifier to cite or link to this item:
Type
ArticleCopyright
Open access
Collections
- IOC - Artigos de Periódicos [12874]
Metadata
Show full item record
EVALUATION OF THE EXPRESSION OF CCR5 AND CX3CR1 RECEPTORS AND CORRELATION WITH THE FUNCTIONALITY OF T CELLS IN WOMEN INFECTED WITH ZIKV DURING PREGNANCY
Gravidez
Células T
Resposta inflamatória
Atividade citotóxica
Receptores de quimiocina
Author
Affilliation
Abstract
There have been reports of neurological abnormalities associated with the Zika virus (ZIKV),
such as congenital Zika syndrome (CZS) in children born to mothers infected during pregnancy. We
investigated how the immune response to ZIKV during pregnancy is primed and conduct a thorough
evaluation of the inflammatory and cytotoxic profiles as well as the expression of CCR5 and CX3CR1.
We compared the reactivity of T cells to ZIKV peptides in convalescent mothers infected during
pregnancy. The child’s clinical outcome (i.e., born with or without CZS) was taken to be the variable.
The cells were stimulated in vitro with ZIKV peptides and evaluated using the ELISPOT and flow
cytometry assays. After in vitro stimulation with ZIKV peptides, we observed a tendency toward
a higher Interferon gamma (IFN-
)-producing T cell responses in mothers who had asymptomatic
children and a higher CD107a expression in T cells in mothers who had children with CZS.We found
a higher frequency of T cells expressing CD107a+ and co-expressing CX3CR1+CCR5+, which is much
clearer in the T cells of mothers who had CZS children. We suggest that this differential profile
influenced the clinical outcome of babies. These data need to be further investigated, including the
evaluation of other ZIKV peptides and markers and functional assays.
Keywords in Portuguese
Zika virusGravidez
Células T
Resposta inflamatória
Atividade citotóxica
Receptores de quimiocina
Share