Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/46122
Title: Results from the WHO external quality assessment for the respiratory syncytial virus pilot, 2016-17
Authors: Jackson, Sandra
Peret, Teresa C. T.
Ziegler, Thedi T.
Thornburg, Natalie J.
Besselaar, Terry
Broor, Shobha
Barr, Ian
Baumeister, Elsa
Chadha, Mandeep
Chittaganpitch, Malinee
Darmaa, Badarch
Ellis, Joanna
Fasce, Rodrigo
Hering, Belinda
Herve, Kadjo
Siddhivinayak, Hirve
Li, Yan
Pisareva, Maria
Moen, Ana
Naguib, Amel
Palekar, Rakee
Potdar, Vasha
Siqueira, Marilda
Treurnicht, Florette
Tivane, Almiro
Venter, Marietjie
Wairagkar, Niteen
Zambom, Maria
Zhang, Wenqing
Affilliation: Global Influenza Programme, World Health Organization. Geneva, Switzerland.
Division of Viral Diseases, Respiratory Viruses Branch, Centers for Disease Control and Prevention (CDC). Atlanta, GA, USA.
Research Center for Child Psychiatry, University of Turku. Turku, Finland.
Division of Viral Diseases, Respiratory Viruses Branch, Centers for Disease Control and Prevention (CDC). Atlanta, GA, USA.
Global Influenza Programme, World Health Organization. Geneva, Switzerland.
Medicine and Health Sciences, Shree Guru Gobind Singh Tricentenaryl University. Gurugram, India.
Victorian Infectious Diseases Reference Laboratory, Peter Doherty Institute for Infection and Immunity. Melbourne, VIC, Australia.
Departamento Virologia, INEI-ANLIS Carlos G Malbrán, Buenos Aires, Argentina,
National Institute of Virology, Indian Council of Medical Research. Pune, India.
Department of Medical Sciences, Ministry of Public Health. Nonthaburi, Thailand.
Virology Laboratory, National Center for Communicable Diseases. Ulanbaatar, Mongolia.
Virus Reference Department, Public Health England. London, UK.
Sub-department of Viral Diseases, Instituto de Salud Pública de Chile. Santiago, Chile.
African Region Office, World Health Organization., Brazzaville, Republic of Congo.
Department of Epidemic Viruses, Institut Pasteur de Côte d’Ivoire. Abidjan, Côte d’Ivoire.
Global Influenza Programme, World Health Organization. Geneva, Switzerland.
Influenza and Respiratory Viruses Section, National Microbiology Laboratory, Public Health Agency of Canada. Winnipeg, MB, Canada.
Laboratory of Molecular Virology, Smorodintsev Research Institute of Influenza. St. Petersburg, Russian Federation.
Global Influenza Programme, World Health Organization. Geneva, Switzerland.
Central Public Health Laboratory, Ministry of Health. Cairo, Egypt.
Pan American Health Organization. Washington, DC, USA.
National Institute of Virology, Indian Council of Medical Research. Pune, India.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Rio de Janeiro, RJ, Brasil.
Centre for Respiratory Diseases and Meningitis, National Institute for Communicable Diseases. Johannesburg, South Africa
Laboratório de Isolamento Viral, Instituto Nacional de Saúde.. Maputo, Mozambique
Center for Viral Zoonosis, Department of Medical Virology.University of Pretoria, Pretoria, South Africa.
Bill and Melinda Gates Foundation. Seattle, WA, USA.
Virus Reference Department, Public Health England. London, UK.
Global Influenza Programme, World Health Organization. Geneva, Switzerland.
Abstract: Background: External quality assessments (EQAs) for the molecular detection of respiratory syncytial virus (RSV) are necessary to ensure the provision of reliable and accurate results. One of the objectives of the pilot of the World Health Organization (WHO) Global RSV Surveillance, 2016-2017, was to evaluate and standardize RSV molecular tests used by participating countries. This paper describes the first WHO RSV EQA for the molecular detection of RSV. Methods: The WHO implemented the pilot of Global RSV Surveillance based on the WHO Global Influenza Surveillance and Response System (GISRS) from 2016 to 2018 in 14 countries. To ensure standardization of tests, 13 participating laboratories were required to complete a 12 panel RSV EQA prepared and distributed by the Centers for Disease Control and Prevention (CDC), USA. The 14th laboratory joined the pilot late and participated in a separate EQA. Laboratories evaluated a RSV rRT-PCR assay developed by CDC and compared where applicable, other Laboratory Developed Tests (LDTs) or commercial assays already in use at their laboratories. Results: Laboratories performed well using the CDC RSV rRT-PCR in comparison with LDTs and commercial assays. Using the CDC assay, 11 of 13 laboratories reported correct results. Two laboratories each reported one false-positive finding. Of the laboratories using LDTs or commercial assays, results as assessed by Ct values were 100% correct for 1/5 (20%). With corrective actions, all laboratories achieved satisfactory outputs. Conclusions: These findings indicate that reliable results can be expected from this pilot. Continued participation in EQAs for the molecular detection of RSV is recommended.
Keywords: RSV external quality assessment
Polymerase chain reaction
Respiratory syncytial viruses
keywords: Avaliação de qualidade externa RSV
Reação em cadeia da polimerase
Vírus sinciciais respiratórios
Issue Date: 2020
Publisher: Wiley
Citation: JACKSON, Sandra et al. Results from the WHO external quality assessment for the respiratory syncytial virus pilot, 2016-17. Influenza Other Respiratory Viruses, v. 14, p. 671-677, 2020.
DOI: 10.1111/irv.12771
ISSN: 1750-2659
Copyright: open access
Appears in Collections:IOC - Artigos de Periódicos
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