| Author | Gomig, Talita Helen Bombardelli | |
| Author | Gontarski, Amanda Moletta | |
| Author | Cavalli, Iglenir João | |
| Author | Souza, Ricardo Lehtonen Rodrigues de | |
| Author | Lucena, Aline Castro Rodrigues | |
| Author | Batista, Michel | |
| Author | Machado, Kelly Cavalcanti | |
| Author | Marchini, Fabrício Klerynton | |
| Author | Marchi, Fabio Albuquerque | |
| Author | Lima, Rubens Silveira | |
| Author | Urban, Cícero de Andrade | |
| Author | Marchi, Rafael Diogo | |
| Author | Cavalli, Luciane Regina | |
| Author | Ribeiro, Enilze Maria de Souza Fonseca | |
| Access date | 2021-03-09T19:32:32Z | |
| Available date | 2021-03-09T19:32:32Z | |
| Document date | 2021 | |
| Citation | GOMIG, Talita Helen Bombardelli et al. Integrated analysis of label-free quantitative proteomics and bioinformatics reveal insights into signaling pathways in male breast cancer. Genetics and Molecular Biology, v. 44, n. 1, p. 1-16, 2021. | pt_BR |
| ISSN | 0100-8455 | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/46316 | |
| Language | por | pt_BR |
| Publisher | Sociedade Brasileira de Genética | pt_BR |
| Rights | open access | pt_BR |
| Title | Integrated analysis of label-free quantitative proteomics and bioinformatics reveal insights into signaling pathways in male breast cancer | pt_BR |
| Type | Article | pt_BR |
| DOI | 10.1590/1678-4685-GMB-2019-0410 | |
| Abstract | Male breast cancer (MBC) is a rare malignancy that accounts for about 1.8% of all breast cancer cases. In contrast to the high number of the “omics” studies in breast cancer in women, only recently molecular approaches have been performed in MBC research. High-throughput proteomics based methodologies are promisor strategies to characterize the MBC proteomic signatures and their association with clinico-pathological parameters. In this study, the label-free quantification-mass spectrometry and bioinformatics approaches were applied to analyze the proteomic profiling of a MBC case using the primary breast tumor and the corresponding axillary metastatic lymph nodes and adjacent non-tumor breast tissues. The differentially expressed proteins were identified in the signaling pathways of granzyme B, sirtuins, eIF2, actin cytoskeleton, eNOS, acute phase response and calcium and were connected to the upstream regulators MYC, PI3K SMARCA4 and cancer-related chemical drugs. An additional proteomic comparative analysis was performed with a primary breast tumor of a female patient and revealed an interesting set of proteins, which were mainly involved in cancer biology. Together, our data provide a relevant data source for the MBC research that can help the therapeutic strategies for its management. | pt_BR |
| Affilliation | Universidade Federal do Paraná. Departamento de Genética. Programa de Pós-Graduação em Genética. Curitiba, PR, Brasil. | pt_BR |
| Affilliation | Universidade Federal do Paraná. Departamento de Genética. Programa de Pós-Graduação em Genética. Curitiba, PR, Brasil. | pt_BR |
| Affilliation | Universidade Federal do Paraná. Departamento de Genética. Programa de Pós-Graduação em Genética. Curitiba, PR, Brasil. | pt_BR |
| Affilliation | Universidade Federal do Paraná. Departamento de Genética. Programa de Pós-Graduação em Genética. Curitiba, PR, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Genômica Funcional. Curitiba, PR, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Genômica Funcional. Curitiba, PR, Brasil / Fundação Oswaldo Cruz. Instituto Carlos Chagas. Plataforma de Espectrometria de Massas. Curitiba, PR, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Carlos Chagas. Plataforma de Espectrometria de Massas. Curitiba, PR, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Genômica Funcional. Curitiba, PR, Brasil / Fundação Oswaldo Cruz. Instituto Carlos Chagas. Plataforma de Espectrometria de Massas. Curitiba, PR, Brasil. | pt_BR |
| Affilliation | Hospital A. C. Camargo. Centro de Pesquisa Internacional. São Paulo, SP, Brasil. | pt_BR |
| Affilliation | Hospital Nossa Senhora das Graças. Centro de Doenças da Mama. Curitiba, PR, Brasil. | pt_BR |
| Affilliation | Hospital Nossa Senhora das Graças. Centro de Doenças da Mama. Curitiba, PR, Brasil. | pt_BR |
| Affilliation | Instituto de Pesquisa Pelé Pequeno Príncipe. Curitiba, PR, Brasil. | pt_BR |
| Affilliation | Instituto de Pesquisa Pelé Pequeno Príncipe. Curitiba, PR, Brasil / Georgetown University. Lombardi Comprehensive Cancer Center. Washington, USA. | pt_BR |
| Affilliation | Universidade Federal do Paraná. Departamento de Genética. Programa de Pós-Graduação em Genética. Curitiba, PR, Brasil. | pt_BR |
| Subject | Breast Neoplasms, Male | pt_BR |
| Subject | Proteomics | pt_BR |
| Subject | Mass Spectrometry | pt_BR |
| Subject | Computational Biology | pt_BR |
| Subject | Signal Transduction | pt_BR |
| Subject in Spanish | Neoplasias de la Mama Masculina | pt_BR |
| Subject in Spanish | Proteómica | pt_BR |
| Subject in Spanish | Espectrometría de Masas | pt_BR |
| Subject in Spanish | Biología Computacional | pt_BR |
| Subject in Spanish | Transducción de Señal | pt_BR |
| Subject in French | Tumeur du sein de l'homme | pt_BR |
| Subject in French | Protéomique | pt_BR |
| Subject in French | Spectrométrie de masse | pt_BR |
| Subject in French | Biologie informatique | pt_BR |
| Subject in French | Transduction du signal | pt_BR |
| DeCS | Neoplasias da Mama Masculina | pt_BR |
| DeCS | Proteômica | pt_BR |
| DeCS | Espectrometria de Massas | pt_BR |
| DeCS | Biologia Computacional | pt_BR |
| DeCS | Transdução de Sinais | pt_BR |
