Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/47629
Title: Circumventing the side effects of L-asparaginase
Authors: Fonseca, Marcela Helena Gambim
Fiúza, Tayná da Silva
Morais, Stephanie Bath de
Souza, Tatiana de Arruda Campos Brasil de
Trevizani, Raphael
Affilliation: Fundação Oswaldo Cruz. Fiocruz Ceará. Eusébio, CE, Brasil.
Núcleo Multidisciplinar de Bioinformática. BioME, RN, Brasil.
Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil.
Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil.
Fundação Oswaldo Cruz. Fiocruz Ceará. Eusébio, CE, Brasil.
Abstract: L-asparaginase is an enzyme that catalyzes the degradation of asparagine and successfully used in the treatment of acute lymphoblastic leukemia. L-asparaginase toxicity is either related to hypersensitivity to the foreign protein or to a secondary L-glutaminase activity that causes inhibition of protein synthesis. PEGylated versions have been incorporated into the treatment protocols to reduce immunogenicity and an alternative L-asparaginase derived from Dickeya chrysanthemi is used in patients with anaphylactic reactions to the E. coli L-asparaginase. Alternative approaches commonly explore new sources of the enzyme as well as the use of protein engineering techniques to create less immunogenic, more stable variants with lower L-glutaminase activity. This article reviews the main strategies used to overcome L-asparaginase shortcomings and introduces recent tools that can be used to create therapeutic enzymes with improved features.
Keywords: Immunogenicity
Immunologic Factors
L-glutaminase activity
Half-Life
Epitope prediction
Deimmunization
Protein Engineering
???metadata.dc.subject.fr???: Facteurs immunologiques
Période
Cartographie épitopique
Ingénierie des protéines
Keywords in spanish: Factores Inmunológicos
Glutaminasa
Semivida
Mapeo Epitopo
Ingeniería de Proteínas
DeCS: Fatores Imunológicos
Glutaminase
Meia-Vida
Mapeamento de Epitopos
Engenharia de Proteínas
Issue Date: 2021
Publisher: Elsevier
Citation: FONSECA, Marcela Helena Gambim et al. Circumventing the side effects of L-asparaginase. Biomedicine & Pharmacotherapy, n. 139, p. 1–7, 2021.
DOI: https://doi.org/10.1016/j.biopha.2021.111616
ISSN: 0753-3322
Copyright: open access
Appears in Collections:PR - ICC - Artigos de Periódicos
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