Please use this identifier to cite or link to this item:
https://www.arca.fiocruz.br/handle/icict/48770
PIPER REGNELLII (MIQ.) C. DC.: CHEMICAL COMPOSITION, ANTIMICROBIAL EFFECTS, AND MODULATION OF ANTIMICROBIAL RESISTANCE
Escherichia coli
Fluconazol
Candida tropicalis
Resistência antimicrobiana
Author
Braga, Ana Lays
Cruz, Rafael Pereira da
Carneiro, Joara Nalyda Pereira
Santos, Antonia Thassya Lucas dos
Sales, Debora Lima
Bezerra, Camila Fonseca
Fonseca, Victor Juno Alencar
Rocha, Janaina Esmeraldo
Freitas, Thiago Sampaio de
Campina, Fabia Ferreira
Costa, Maria do Socorro
Amaral, Wanderlei do
Rebelo, Ricardo Andrade
Silva, Luiz Everson da
Ribeiro Filho, Jaime
Coutinho, Henrique Douglas Melo
Rodriguese, Fabíola Fernandes Galvão
Braga, Maria Flaviana Bezerra Morais
Cruz, Rafael Pereira da
Carneiro, Joara Nalyda Pereira
Santos, Antonia Thassya Lucas dos
Sales, Debora Lima
Bezerra, Camila Fonseca
Fonseca, Victor Juno Alencar
Rocha, Janaina Esmeraldo
Freitas, Thiago Sampaio de
Campina, Fabia Ferreira
Costa, Maria do Socorro
Amaral, Wanderlei do
Rebelo, Ricardo Andrade
Silva, Luiz Everson da
Ribeiro Filho, Jaime
Coutinho, Henrique Douglas Melo
Rodriguese, Fabíola Fernandes Galvão
Braga, Maria Flaviana Bezerra Morais
Affilliation
"Múltipla ver em Notas"
Abstract
Plant-derived essential oils are volatile hydrophobic compounds with significant antimicrobial activities.
Considering the rise of antimicrobial resistance, these natural products have been highlighted as efficient
weapons against multidrug-resistant microorganisms. This work aimed to investigate the phytochemical
composition and antimicrobial activity of Piper regnellii essential oil against strains of Escherichia coli, Staphylococcus
aureus, Candida albicans, and Candida tropicalis. Phytochemical analysis was performed through gas
chromatography coupled with mass spectrometry (CG/MS). The intrinsic antimicrobial activity and the ability
of the essential oil to modulate antimicrobial resistance were assessed using the broth microdilution
method. Fungal virulence inhibition was analyzed by measuring the growth of hyphae in microculture chambers.
Phytochemical characterization revealed a predominance of phenylpropanoids, including apiol (70.79%)
and dilapiol (15.05%) as major constituents. While presenting clinically ineffective antibacterial effects (MIC
1.024 mg/mL for all strains), the essential oil potentiated the activity of gentamicin against E. coli at concentrations
above 20 mg/mL. Piper regnellii essential oil showed clinically ineffective antifungal activity with IC50
values above 500 mg/mL. However, it was found to potentiate the activity of fluconazole against C. tropicalis
at concentrations ranging from 32 mg/mL to 1024 mg/mL. Furthermore, the morphological transition was
inhibited by culturing C. albicans and C. tropicalis with different concentrations of the essential oil. Together,
our results indicate that P. regnellii essential oil presents promising antifungal effects. However, the mechanisms
underlying its interference on Candida virulence remain to be further investigated.
Keywords in Portuguese
Piper regnelliiEscherichia coli
Fluconazol
Candida tropicalis
Resistência antimicrobiana
Share