Author | Bombaça, Ana Cristina S. | |
Author | Gandara, Ana Caroline P. | |
Author | Ennes-Vidal, Vitor | |
Author | Bottino-Rojas, Vanessa | |
Author | Dias, Felipe A. | |
Author | Farnesi, Luana C. | |
Author | Sorgine, Marcos H. | |
Author | Bahia, Ana Cristina | |
Author | Bruno, Rafaela V. | |
Author | Menna-Barreto, Rubem F. S. | |
Access date | 2021-10-03T12:03:28Z | |
Available date | 2021-10-03T12:03:28Z | |
Document date | 2021 | |
Citation | BOMBAÇA, Ana Cristina S. et al. Aedes aegypti Infection With Trypanosomatid Strigomonas culicis Alters Midgut Redox Metabolism and Reduces Mosquito Reproductive Fitness. Frontiers in Cellular and Infection Microbiology, v. 11, Article 732925, p. 1-17, Aug. 2021. | pt_BR |
ISSN | 2235-2988 | pt_BR |
URI | https://www.arca.fiocruz.br/handle/icict/49237 | |
Language | eng | pt_BR |
Publisher | Frontiers Media | pt_BR |
Rights | open access | |
Subject in Portuguese | Aedes aegypti | pt_BR |
Subject in Portuguese | Strigomonas culicis | pt_BR |
Subject in Portuguese | Oxidases Duais | pt_BR |
Subject in Portuguese | Acatalasia | pt_BR |
Subject in Portuguese | Espécies Reativas de Oxigênio | pt_BR |
Subject in Portuguese | Fecundidade | pt_BR |
Subject in Portuguese | Fertilidade | pt_BR |
Title | Aedes aegypti Infection With Trypanosomatid Strigomonas culicis Alters Midgut Redox Metabolism and Reduces Mosquito Reproductive Fitness | pt_BR |
Type | Article | |
DOI | 10.3389/fcimb.2021.732925 | |
Abstract | Aedes aegypti mosquitoes transmit arboviruses of important global health impact, and their
intestinal microbiota can influence vector competence by stimulating the innate immune
system. Midgut epithelial cells also produce toxic reactive oxygen species (ROS) by dual
oxidases (DUOXs) that are essential players in insect immunity. Strigomonas culicis is a
monoxenous trypanosomatid that naturally inhabits mosquitoes; it hosts an endosymbiotic
bacterium that completes essential biosynthetic pathways of the parasite and influences its
oxidative metabolism. Our group previously showed that S. culicis hydrogen peroxide
(H2O2)-resistant (WTR) strain is more infectious to A. aegypti mosquitoes than the wild-type
(WT) strain. Here, we investigated the influence of both strains on the midgut oxidative
environment and the effect of infection onmosquito fitness and immunity.WT stimulated the
production of superoxide by mitochondrial metabolism of midgut epithelial cells after 4 days
post-infection, while WTR exacerbated H2O2 production mediated by increased DUOX
activity and impairment of antioxidant system. The infection with both strains also disrupted
the fecundity and fertility of the females, with a greater impact on reproductive fitness of
WTR-infected mosquitoes. The presence of these parasites induced specific transcriptional
modulation of immune-related genes, such as attacin and defensin A during WTR infection
(11.8- and 6.4-fold, respectively) and defensin C in WT infection (7.1-fold). Thus, we
propose that A. aegypti oxidative response starts in early infection time and does not
affect the survival of the H2O2-resistant strain, which has a more efficient antioxidant system.
Our data provide new biological aspects of A. aegypti–S. culicis relationship that can be
used later in alternative vector control strategies. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Insituto Oswaldo Cruz. Laboratório de Biologia Celular. Rio de Janeiro, RJ, Brasil. | pt_BR |
Affilliation | Universidade Federal do Rio de Janeiro. Instituto de Bioquímica Médica. Laboratório de Bioquímica de Artrópodes Hematófagos. Rio de Janeiro, RJ, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Insituto Oswaldo Cruz. Laboratório de Estudos Integrados em Protozoologia. Rio de Janeiro, RJ, Brasil. | pt_BR |
Affilliation | Universidade Federal do Rio de Janeiro. Instituto de Bioquímica Médica. Laboratório de Bioquímica de Artrópodes Hematófagos. Rio de Janeiro, RJ, Brasil. | pt_BR |
Affilliation | Universidade Federal do Rio de Janeiro. Instituto de Bioquímica Médica. Laboratório de Bioquímica de Artrópodes Hematófagos. Rio de Janeiro, RJ, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Insituto Oswaldo Cruz. Laboratório de Biologia Molecular de Insetos. Rio de Janeiro, RJ, Brasil. | pt_BR |
Affilliation | Universidade Federal do Rio de Janeiro. Instituto de Bioquímica Médica. Laboratório de Bioquímica de Artrópodes Hematófagos. Rio de Janeiro, RJ, Brasil. | pt_BR |
Affilliation | Universidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho. Laboratório de Bioquímica de Insetos e Parasitos. Rio de Janeiro, RJ, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Insituto Oswaldo Cruz. Laboratório de Biologia Molecular de Insetos. Rio de Janeiro, RJ, Brasil / Instituto Nacional de Ciência e Tecnologia em Entomologia Molecular (INCT-EM/CNPq), Rio de Janeiro, RJ, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Insituto Oswaldo Cruz. Laboratório de Biologia Celular. Rio de Janeiro, RJ, Brasil. | pt_BR |
Subject | Aedes aegypti | pt_BR |
Subject | Strigomonas culicis | pt_BR |
Subject | Dual oxidase | pt_BR |
Subject | Catalase | pt_BR |
Subject | Reactive oxygen species | pt_BR |
Subject | Fecundity | pt_BR |
Subject | Fertility | pt_BR |
DeCS | Oxidases Duais | pt_BR |
DeCS | Acatalasia | pt_BR |
DeCS | Espécies Reativas de Oxigênio | pt_BR |