Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/50054
Title: Metacyclogenesis defects and gene expression hallmarks of histone deacetylase 4‑deficient Trypanosoma cruzi cells
Authors: Picchi‑Constante, Gisele Fernanda Assine
Guerra‑Slompo, Eloise Pavão
Tahira, Ana Carolina
Alcantara, Monica Visnieski
Amaral, Murilo Sena
Ferreira, Arthur Schveitzer
Batista, Michel
Batista, Cassiano Martin
Goldenberg, Samuel
Verjovski‑Almeida, Sergio
Zanchin, Nilson Ivo Tonin
Affilliation: Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil.
Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil.
Instituto Butantan. Laboratório de Parasitologia. São Paulo, SP, Brasil.
Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil.
Instituto Butantan. Laboratório de Parasitologia. São Paulo, SP, Brasil.
Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil.
Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil.
Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil.
Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil.
Instituto Butantan. Laboratório de Parasitologia. São Paulo, SP, Brasil. / Universidade de São Paulo. Instituto de Química. Departamento de Bioquímica. São Paulo, SP, Brasil.
Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil.
Abstract: Trypanosoma cruzi—the causative agent of Chagas disease—like other kinetoplastids, relies mostly on post-transcriptional mechanisms for regulation of gene expression. However, trypanosomatids undergo drastic changes in nuclear architecture and chromatin structure along their complex life cycle which, combined with a remarkable set of reversible histone post-translational modifcations, indicate that chromatin is also a target for control of gene expression and diferentiation signals in these organisms. Chromatin-modifying enzymes have a direct impact on gene expression programs and DNA metabolism. In this work, we have investigated the function of T. cruzi histone deacetylase 4 (TcHDAC4). We show that, although TcHDAC4 is not essential for viability, metacyclic trypomastigote TcHDAC4 null mutants show a thin cell body and a round and less condensed nucleus located very close to the kinetoplast. Sixty-four acetylation sites were quantitatively evaluated, which revealed H2AT85ac, H4K10ac and H4K78ac as potential target sites of TcHDAC4. Gene expression analyses identifed three chromosomes with overrepresented regions of diferentially expressed genes in the TcHDAC4 knockout mutant compared with the wild type, showing clusters of either up or downregulated genes. The adjacent chromosomal location of some of these genes indicates that TcHDAC4 participates in gene expression regulation during T. cruzi diferentiation.
Keywords: Gene Expression Regulation
Histone Deacetylases
Cell Differentiation
???metadata.dc.subject.fr???: Régulation de l'expression des gènes
Histone deacetylases
Différenciation cellulaire
Keywords in spanish: Regulación de la Expresión Génica
Histona Desacetilasas
Diferenciación Celular
DeCS: Trypanosoma cruzi
Regulação da Expressão Gênica
Histona Desacetilases
Diferenciação Celular
Issue Date: 2021
Publisher: Nature Research
Citation: PICCHI-CONSTANTE, Gisele Fernanda Assine et al. Metacyclogenesis defects and gene expression hallmarks of histone deacetylase 4‑deficient Trypanosoma cruzi cells. Scientific Reports, v.11, n. 21671, p. 1–18, 2021.
DOI: 10.1038/s41598-021-01080-1
ISSN: 2045-2322
Copyright: open access
Appears in Collections:PR - ICC - Artigos de Periódicos
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