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ANTIOXIDANT ACTIVITY OF STRYPHNODENDRON ROTUNDIFOLIUM MART. STEM BARK FRACTION IN AN IRON OVERLOAD MODEL
Radicais livres
Sobrecarga de ferro
Stryphnodendron
Taninos
Free radicals
Iron overload
Stryphnodendron rotundifolium
Tannins
Autor
Afiliación
Regional University of Cariri. Postgraduate Program in Ethnobiology and Nature Conservation. Crato, CE, Brazil.
Regional University of Cariri. Postgraduate Program in Ethnobiology and Nature Conservation. Crato, CE, Brazil.
Embrapa Tropical Agroindustry. Multi-User Laboratory of Natural Products Chemistry. Fortaleza, CE, Brazil.
Embrapa Tropical Agroindustry. Multi-User Laboratory of Natural Products Chemistry. Fortaleza, CE, Brazil.
Embrapa Tropical Agroindustry. Multi-User Laboratory of Natural Products Chemistry. Fortaleza, CE, Brazil.
Regional University of Cariri. Postgraduate Program in Biological Chemistry. Crato, CE, Brazil.
Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Laboratório de Investigação em Genética e Hematologia Translacional. Salvador, BA, Brasil.
University of Naples Federico II. Department of Molecular Medicine and Medical Biotechnology. Naples, Italy.
University of Naples Federico II. Department of Pharmacy. Naples, Italy.
University of Naples Federico II. Department of Chemical Sciences. Naples, Italy.
Selcuk University. Department of Biology, Science Faculty. Konya, Turkey.
Regional University of Cariri. Postgraduate Program in Ethnobiology and Nature Conservation. Crato, CE, Brazil / Regional University of Cariri. Postgraduate Program in Biological Chemistry. Crato, CE, Brazil.
Regional University of Cariri. Postgraduate Program in Ethnobiology and Nature Conservation. Crato, CE, Brazil.
Embrapa Tropical Agroindustry. Multi-User Laboratory of Natural Products Chemistry. Fortaleza, CE, Brazil.
Embrapa Tropical Agroindustry. Multi-User Laboratory of Natural Products Chemistry. Fortaleza, CE, Brazil.
Embrapa Tropical Agroindustry. Multi-User Laboratory of Natural Products Chemistry. Fortaleza, CE, Brazil.
Regional University of Cariri. Postgraduate Program in Biological Chemistry. Crato, CE, Brazil.
Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Laboratório de Investigação em Genética e Hematologia Translacional. Salvador, BA, Brasil.
University of Naples Federico II. Department of Molecular Medicine and Medical Biotechnology. Naples, Italy.
University of Naples Federico II. Department of Pharmacy. Naples, Italy.
University of Naples Federico II. Department of Chemical Sciences. Naples, Italy.
Selcuk University. Department of Biology, Science Faculty. Konya, Turkey.
Regional University of Cariri. Postgraduate Program in Ethnobiology and Nature Conservation. Crato, CE, Brazil / Regional University of Cariri. Postgraduate Program in Biological Chemistry. Crato, CE, Brazil.
Resumen en ingles
Stryphnodendron rotundifolium Mart., popularly known as “barbatimão”, is a plant species
traditionally used by topical and oral routes for the treatment of infectious and inflammatory diseases.
Considering the well-described antioxidant properties of this species, this study investigated the
protective effects of its keto-aqueous extract using an in vitro model of iron overload. Phenolic compounds
were quantified and identified by Ultra-Performance Liquid Chromatography coupled with
quadrupole Time-Of-Flight Electrospray Ionization Mass Spectrometry (UPLC–ESI-qTOF-MS/MS)
in positive and negative ions mode analysis. Antioxidant activity was analyzed following the
iron-chelating–reducing capacity and deoxyribose degradation (2-DR) protection methods. The
analysis identified condensed tannins (54.8 mg catechin/g dry fraction (DF), polyphenols (25 mg
gallic acid/g DF), and hydrolyzable tannins (28.8 mg tannic acid/g DF). Among the constituents,
prodelphinidin, procyanidin, and prorobinetinidine were isolated and identified. The extract significantly
protected 2-DR degradation induced by Fe2+ (72% protection) or OH (43% protection).
The ortho-phenanthroline test revealed Fe2+-chelating and Fe3+-reducing activities of 93% and 84%,
respectively. A preliminary toxicological analysis using Artemia salina revealed mortality below 10%,
at a concentration of 0.25 mg/mL, indicating low toxicity under the present experimental conditions.
In conclusion, the findings of the present study indicate that Stryphnodendron rotundifolium is a source
of antioxidant compounds with the potential to be used in drug development in the context of iron
overload disorders, which remains to be further investigated in vivo.
Palabras clave en portugues
AntioxidantesRadicais livres
Sobrecarga de ferro
Stryphnodendron
Taninos
Palabras clave en ingles
Antioxidant activityFree radicals
Iron overload
Stryphnodendron rotundifolium
Tannins
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