Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/50757
Title: A Large Cohort Study of SARS-CoV-2 Detection in Saliva: A Non-Invasive Alternative Diagnostic Test for Patients with Bleeding Disorders
Authors: Lopes, Josiane Iole França
Silva, Carlos Alexandre da Costa
Cunha, Rodrigo Guimarães
Soares, Alexandra Martins
Lopes, Maria Esther Duarte
Conceição Neto, Orlando Carlos da
Alves, Arthur Daniel Rocha
Coelho, Wagner Luis da Costa Nunes Pimentel
Amorim Filho, Luiz
Leon, Luciane Almeida Amado
Affilliation: Instituto de Hematologia Arthur de Siqueira Cavalcanti/Hemorio.Rio de Janeiro, RJ, Brasil / Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Desenvolvimento Tecnológico em Virologia. Rio de Janeiro, RJ, Brasil.
Instituto de Hematologia Arthur de Siqueira Cavalcanti/Hemorio.Rio de Janeiro, RJ, Brasil.
Instituto de Hematologia Arthur de Siqueira Cavalcanti/Hemorio.Rio de Janeiro, RJ, Brasil.
Instituto de Hematologia Arthur de Siqueira Cavalcanti/Hemorio.Rio de Janeiro, RJ, Brasil.
Instituto de Hematologia Arthur de Siqueira Cavalcanti/Hemorio.Rio de Janeiro, RJ, Brasil.
Instituto de Hematologia Arthur de Siqueira Cavalcanti/Hemorio.Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Desenvolvimento Tecnológico em Virologia. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Desenvolvimento Tecnológico em Virologia. Rio de Janeiro, RJ, Brasil.
Instituto de Hematologia Arthur de Siqueira Cavalcanti/Hemorio.Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Desenvolvimento Tecnológico em Virologia. Rio de Janeiro, RJ, Brasil.
Abstract: Diagnosis of SARS-CoV-2 infections is mostly based on the nasopharyngeal swabs (NPS). However, this collection is invasive and uncomfortable, especially for children and patients with coagulopathies, whose NPS collection often causes bleeding. Thus, the aim of this study was to evaluate the usefulness and accuracy of saliva for the diagnosis of COVID-19 in patients presenting bleeding disorders. Samples of NPS, oropharyngeal swabs (OPS), and saliva were collected simultaneously from 1159 hospitalized patients with hematological diseases and from 524 healthcare workers, both symptomatic and asymptomatic for SARS-CoV-2. All samples were evaluated for SARS-CoV-2 by qRT-PCR. SARS-CoV-2 was detected in NPS, OPS and saliva from 16.9%, 14.4% and 15.6% individuals, respectively. Tests in saliva showed sensitivity, specificity, and overall agreement of 73.3%, 96.9% and 92.7% (=0.74), respectively. Salivary tests had good accuracy (AUC = 0.7) for discriminating negative and positive qRT-PCR for SARS-CoV-2. Higher sensitivity was observed in symptomatic than in non-symptomatic patients, as well as in healthy subjects than in patients with hematological disease, in both OPS and saliva. The mean viral load in NPS was significantly higher than in OPS and in saliva samples (p < 0.001). Saliva is a good diagnostic tool to detect SARS-CoV-2, especially among patients symptomatic for COVID-19, and is a valuable specimen for mass screening of hospitalized patients with hematological diseases, especially for those that with bleeding disorders.
Keywords: Saliva
SARS-CoV-2
COVID-19
Diagnosis
Hematological disease
Bleeding disorders
keywords: Saliva
SARS-CoV-2
COVID-19
Diagnóstico
Doença hematológica
Distúrbios hemorrágicos
Issue Date: 2021
Publisher: MDPI
Citation: LOPES, Josiane Iole França et al. A Large Cohort Study of SARS-CoV-2 Detection in Saliva: A Non-Invasive Alternative Diagnostic Test for Patients with Bleeding Disorders. Viruses, v. 13, 2361, p. 1- 11, Nov. 2021.
DOI: 10.3390/v13122361
ISSN: 1999-4915
Copyright: open access
Appears in Collections:IOC - Artigos de Periódicos
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