| Author | Azamor, Tamiris | |
| Author | Silva, Andréa Marques Vieira da | |
| Author | Melgaço, Juliana Gil | |
| Author | Santos, Ana Paula dos | |
| Author | Carvalho, Caroline Xavier | |
| Author | Alvarado-Arnez, Lucia Elena | |
| Author | Silva, Leonardo Ribeiro Batista | |
| Author | Matos, Denise Cristina de Souza | |
| Author | Bayma, Camilla | |
| Author | Missailidis, Sotiris | |
| Author | Ano Bom, Ana Paula Dinis | |
| Author | Moraes, Milton Ozório | |
| Author | Neves, Patrícia Cristina da Costa | |
| Access date | 2022-02-09T19:38:41Z | |
| Available date | 2022-02-09T19:38:41Z | |
| Document date | 2021 | |
| Citation | AZAMOR, Tamiris et al. Activation of an Effective Immune Response after Yellow Fever Vaccination Is Associated with the Genetic Background and Early Response of IFN- and CLEC5A. Viruses, v. 13, n. 96, p. 1 - 12, Jan. 2021. | pt_BR |
| ISSN | 1999-4915 | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/51125 | |
| Language | eng | pt_BR |
| Publisher | MDPI | pt_BR |
| Rights | open access | pt_BR |
| Subject in Portuguese | Febre Amarela | pt_BR |
| Subject in Portuguese | Vacina | pt_BR |
| Subject in Portuguese | Interferon gamma | pt_BR |
| Title | Activation of an Effective Immune Response after Yellow Fever Vaccination Is Associated with the Genetic Background and Early Response of IFN- and CLEC5A | pt_BR |
| Type | Article | pt_BR |
| DOI | 10.3390/ v13010096 | |
| Abstract | The yellow fever vaccine (YF17DD) is highly effective with a single injection conferring
protection for at least 10 years. The YF17DD induces polyvalent responses, with a TH1/TH2 CD4+
profile, robust T CD8+ responses, and synthesis of interferon-gamma (IFN-
), culminating in high
titers of neutralizing antibodies. Furthermore, C-type lectin domain containing 5A (CLEC5A) has
been implicated in innate outcomes in other flaviviral infections. Here, we conducted a followup
study in volunteers immunized with YF17DD, investigating the humoral response, cellular
phenotypes, gene expression, and single nucleotide polymorphisms (SNPs) of IFNG and CLEC5A,
to clarify the role of these factors in early response after vaccination. Activation of CLEC5A+
monocytes occurred five days after vaccination (DAV). Following, seven DAV data showed activation
of CD4+ and CD8+T cells together with early positive correlations between type II IFN and genes
of innate antiviral response (STAT1, STAT2, IRF7, IRF9, OAS1, and RNASEL) as well as antibody
levels. Furthermore, individuals with genotypes rs2430561 AT/AA, rs2069718 AG/AA (IFNG),
and rs13237944 AC/AA (CLEC5A), exhibited higher expression of IFNG and CLEC5A, respectively.
Together, we demonstrated that early IFN-
and CLEC5A responses, associated with rs2430561,
rs2069718, and rs13237944 genotypes, may be key mechanisms in the long-lasting immunity elicited
by YF17DD. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Bio-Manguinhos. Rio de Janeiro, RJ, Brasil / Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Bio-Manguinhos. Rio de Janeiro, RJ, Brasil | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Bio-Manguinhos. Rio de Janeiro, RJ, Brasil | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Bio-Manguinhos. Rio de Janeiro, RJ, Brasil | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | National Research Coordination, Universidad Privada Franz Tamayo. Cochabamba 4780, Bolivia. | pt_BR |
| Affilliation | Instituto Nacional do Câncer. Coordenação de Pesquisa. Programa de Carcinogênese Molecular. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Bio-Manguinhos. Rio de Janeiro, RJ, Brasil | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Bio-Manguinhos. Rio de Janeiro, RJ, Brasil | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Bio-Manguinhos. Rio de Janeiro, RJ, Brasil | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Bio-Manguinhos. Rio de Janeiro, RJ, Brasil | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Bio-Manguinhos. Rio de Janeiro, RJ, Brasil | pt_BR |
| Subject | Yellow fever | pt_BR |
| Subject | Vaccine | pt_BR |
| Subject | Interferon gamma | pt_BR |
| Subject | CLEC5A | pt_BR |
