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ESTUDOS SOBRE A DOSAGEM MICROBIOLOGICA DAS VITAMINAS DO COMPLEXO B - II. NIACINA
Alternative title
Microbiological assay for niacineAffilliation
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Rio de Janeiro, RJ, Brasil.
Instituto de Biofísica da Universidade do Brasil. Rio de janeiro, RJ, Brasil.
Instituto de Biofísica da Universidade do Brasil. Rio de janeiro, RJ, Brasil.
Abstract
The microbiological assay method of Snell and Wright for niacine was studied and some modifications of the basal medium were proposed. A maximal growth of the "Lactobacillus arabinosus" was obtained by the addition to the basal medium of 25 mg % asparagine and increasing the percentages of glucose and sodium acetate. Liver and yeast extracts were assayed satisfactory and the niacine added was recovered quantitatively.
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