Author | Donadio, Flávia F. | |
Author | Siqueira, Marilda Agudo Mendonça Teixeira de | |
Author | Vyse, Andrew | |
Author | Jin, Lin | |
Author | Oliveira, Solange A. | |
Access date | 2022-03-08T19:44:33Z | |
Available date | 2022-03-08T19:44:33Z | |
Document date | 2003 | |
Citation | DONADIO, Flávia F. et al. The genomic analysis of rubella virus detected from outbreak and sporadic cases in Rio de Janeiro state, Brazil. Journal of Clinical Virology, v. 27, p. 205-209, Nov. 2003. | pt_BR |
ISSN | 1873-5967 | pt_BR |
URI | https://www.arca.fiocruz.br/handle/icict/51626 | |
Language | eng | pt_BR |
Publisher | Elsevier | pt_BR |
Rights | open access | |
Subject in Portuguese | Vírus da Rubéola | pt_BR |
Subject in Portuguese | Epidemiologia molecular | pt_BR |
Subject in Portuguese | Análise genômica | pt_BR |
Subject in Portuguese | Surto | pt_BR |
Subject in Portuguese | Rio de Janeiro | pt_BR |
Subject in Portuguese | Brasil | pt_BR |
Title | The genomic analysis of rubella virus detected from outbreak and sporadic cases in Rio de Janeiro state, Brazil | pt_BR |
Type | Article | pt_BR |
DOI | 10.1016/S1386-6532(02)00270-6 | |
Abstract | Background: The molecular epidemiology of rubella virus (RV) based on the analysis of the viral E1 gene sequences
indicated the existence of two genotypes that differ from each other by 8 to 10% in their nucleotide sequences: genotype
I is present in Europe, North America and Asia; and genotype II is present only in Asia. Objectives: The purpose of the
study was to identify the RV genotypes circulating in Brazil. Study design: In this study, we analysed 86 clinical samples
collected between 1996 and 1999 during a rubella outbreak and from sporadic cases of rubella in Rio de Janeiro State.
For the molecular characterisation of RV strains we have used PCR/nested amplification and direct sequencing of a
513-nucleotide region of the E1 gene. Results: The E1 gene sequences of 14 RVs were obtained and were assigned to
two lineages, both within genotype I. The percentage divergence of nucleotide sequence ranged from 3.4 to 5.1%
between these two lineages. These results were in agreement with the pattern of variation observed among the sequences
obtained from other lineages of RV. Conclusions: This work demonstrated that two new lineages of RV circulated
simultaneously between the years 1996 and 1999 in the state of Rio de Janeiro. These results provided new approaches
for monitoring the progress of vaccination efforts in Brazil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Virologia. Rio de Janeiro, RJ, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Virologia. Rio de Janeiro, RJ, Brasil. | pt_BR |
Affilliation | Enteric, Respiratory and Neurological Virus Laboratory, Central Public Health Laboratory. London NW9 5HT, UK. | pt_BR |
Affilliation | Enteric, Respiratory and Neurological Virus Laboratory, Central Public Health Laboratory. London NW9 5HT, UK. | pt_BR |
Affilliation | Universidade Federal Fluminense. Hospital Universitário Antonio Pedro. Departamento de Doenças Infecciosas. Niterói, RJ, Brasil. | pt_BR |
Subject | Genomic analysis | pt_BR |
Subject | Molecular epidemiology | pt_BR |
Subject | Outbreak | pt_BR |
Subject | Genotype | pt_BR |
Subject | Rio de Janeiro state | pt_BR |
Subject | Brazil | pt_BR |
Subject | Rubella virus | pt_BR |