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REPLACEMENT OF ANIMALS USED IN THE MAINTENANCE OF AEDES AEGYPTI BY ALTERNATIVE METHODS. REFINEMENT OF PROCEDURES THAT MINIMIZE THE DISCOMFORT OF INSECT MAINTENANCE BY SWISS WEBSTER MICE
Affilliation
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Celular. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Doenças Parasitárias. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Doenças Parasitárias. Rio de Janeiro, RJ, Brasil.
Abstract
Meal) and breeding of hematophagous insects, vectors of serious and (often) neglected diseases. In summary, the main objectives
of the project are:
a) Replacement of the use of mice (Mus musculus) for the blood meal of Aedes aegypti in the laboratory.
b) In case of failure to replace the animal, that is, in all phases of the life cycle of Aedes aegypti, we will test the application of two
refinements in the procedure: The first is based on the use of Erythropoietin, increasing the number of and we hope to speed up the
time to food satiety for the mosquito and the second to use an analgesic, an opiate with minimal adverse effects. Complementing, in
the case of successful use of Erythropoietin. We will apply the association of the recombinant hormone with the analgesic for mice
during the Aedes aegypti Blood Meal in the laboratory aiming, firstly, to avoid the use of the animal. However, if it is not possible to
apply it in the maintenance routine of Aedes aegypti to minimize the stress and discomfort of this animal during feeding and creation
of hematophagous vectors, in our case, the mosquito.
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