Potato apyrase reduces granulomatous area and increases presence of multinucleated giant cells in murine schistosomiasis

Department of Biochemistry. Department of Parasitology, Microbiology and Immunology. Institute of Biological Sciences. Federal University of Juiz de Fora. Juiz de Fora, MG, Brazil.
Department of Biochemistry. Department of Parasitology, Microbiology and Immunology. Institute of Biological Sciences. Federal University of Juiz de Fora. Juiz de Fora, MG, Brazil.
Department of Biochemistry. Department of Parasitology, Microbiology and Immunology. Institute of Biological Sciences. Federal University of Juiz de Fora. Juiz de Fora, MG, Brazil.
Department of Biochemistry. Department of Parasitology, Microbiology and Immunology. Institute of Biological Sciences. Federal University of Juiz de Fora. Juiz de Fora, MG, Brazil.
Department of Biochemistry. Department of Parasitology, Microbiology and Immunology. Institute of Biological Sciences. Federal University of Juiz de Fora. Juiz de Fora, MG, Brazil.
University Hospital. Federal University of Juiz de Fora. Juiz de Fora, MG, Brazil.
René Rachou Institute. Oswaldo Cruz Foundation. Belo Horizonte, MG, Brazil.
René Rachou Institute. Oswaldo Cruz Foundation. Belo Horizonte, MG, Brazil.
Department of Biochemistry. Department of Parasitology, Microbiology and Immunology. Institute of Biological Sciences. Federal University of Juiz de Fora. Juiz de Fora, MG, Brazil.
Department of Pharmaceutical Science. Faculty of Pharmacy. Federal University of Juiz de Fora. Juiz de Fora, MG, Brazil.
Department of Biochemistry. Department of Parasitology, Microbiology and Immunology. Institute of Biological Sciences. Federal University of Juiz de Fora. Juiz de Fora, MG, Brazil.
Department of Biochemistry. Department of Parasitology. Microbiology and Immunology. Institute of Biological Sciences. Federal University of Juiz de Fora. Juiz de Fora, MG,Brasil

Resumo em Inglês

Granulomas are inflammatory tissue responses directed to a set of antigens. Trapped Schistosoma mansoni eggs promote productive granulomas in the tissues, and they are the main damage caused by schistosomiasis. Some S. mansoni antigenic proteins may have a direct involvement in the resolution of the granulomatous response. The ATP diphosphohydrolases isoforms of this parasite are immunogenic, expressed in all phases of the parasite life cycle and secreted by eggs and adult worms. Potato apyrase is a vegetable protein that cross-reactive with parasite ATP diphosphohydrolases isoforms. In this study, the vegetable protein was purified, before being inoculated in C57BL/6 mice that were later infected with cercariae. Sixty days after infection, adult worms were recovered, antibodies and cytokines were measured, and morphological granuloma alterations evaluated. Immunization of the animals induced significant levels of IgG and IgG1 antibodies and IFN-γ, IL-10 and IL-5 cytokines, but not IL-13, suggesting that potato apyrase is an immunoregulatory protein. Supporting this hypothesis, it was found that liver damage associated with schistosomiasis was mitigated, reducing the size of the areas affected by granuloma to 35% and increasing the presence of multinucleated giant cells in this environment. In conclusion, potato apyrase was found to be effective immunomodulatory antigen for murine schistosomiasis.

Palavras-chave em inglês

ATP diphosphohydrolase
Cross reactivity
Immunomodulation
NTPDase
Schistosoma mansoni

Editor

Elsevier

Referência

GUSMÃO, Michélia Antônia do Nascimento et al. Potato apyrase reduces granulomatous area and increases presence of multinucleated giant cells in murine schistosomiasis. Parasitol Int., v. 83, 102317, 2021. doi: 10.1016/j.parint.2021.102317.

DOI

10.1016/j.parint.2021.102317

ISSN

1383-5769

Use este identificador para citar ou linkar para este item: https://www.arca.fiocruz.br/handle/icict/51773

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