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METABOLIC ALTERATION OF TRYPANOSOMA CRUZI DURING DIFFERENTIATION OF EPIMASTIGOTE TO TRYPOMASTIGOTE FORMS
Metabolismo de carboidratos
Ácido orgânico
Cromatografia líquida de alta performance
Atividade enzimática
Ácido carboxílico
Organic acid
High-performance liquid chromatography
Enzyme activity
Carboxylic acid
Trypanosoma cruzi
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Abstract
Intracellular parasites such as Trypanosoma cruzi need to acquire valuable carbon sources
from the host cell to replicate. Here, we investigated the energetic metabolism of T. cruzi during
metacyclogenesis through the determination of enzymatic activities and quantification by HPLC of
glycolytic and Krebs cycle short-chain carboxylic acids. Altered concentrations in pyruvate, acetate,
succinate, and glycerate were measured during the growth of epimastigote in the complex medium
BHI and their differentiation to trypomastigotes in the chemically defined medium, TAU3AAG. These
alterations should represent significant differential metabolic modifications utilized by either form to
generate energy. This paper is the first work dealing with the intracellular organic acid concentration
measurement in T. cruzi parasites. Although it confirms the previous assumption of the importance
of carbohydrate metabolism, it yields an essential improvement in T. cruzi metabolism knowledge.
Keywords in Portuguese
Trypanosoma cruziKeywords
Trypanosoma cruziMetabolismo de carboidratos
Ácido orgânico
Cromatografia líquida de alta performance
Atividade enzimática
Ácido carboxílico
Keywords in Spanish
Sarbohydrate metabolismOrganic acid
High-performance liquid chromatography
Enzyme activity
Carboxylic acid
Trypanosoma cruzi
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