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ACINETOBACTER CALCOACETICUS-ACINETOBACTER BAUMANNII COMPLEX IN ANIMALS: IDENTIFICATION AND ANTIMICROBIAL RESISTANCE PROFILE
Holmström, Thérèsse C.H. et al. | Date Issued: 2022
Author
Holmström, Thérèsse C.H.
David, Luria A.
Motta, Cássia C.
Souza, Claudio M. Rocha de
Maboni, Grazieli
Coelho, Irene S.
Melo, Dayanne A.
Souza, Miliane M.S.
Affilliation
Universidade Federal Rural do Rio de Janeiro. Instituto de Veterinária. Departamento de Microbiologia e Imunologia Veterinária. Laboratório de Bacteriologia Veterinária (LABACVET). Seropédica, RJ, Brasil.
Universidade Federal Rural do Rio de Janeiro. Instituto de Veterinária. Departamento de Microbiologia e Imunologia Veterinária. Laboratório de Bacteriologia Veterinária (LABACVET). Seropédica, RJ, Brasil.
Vet Análises – Laboratório Veterinário. Laranjeiras, Rio de Janeiro, RJ.
FUndação Oswaldo Cruz. Laboratório de Pesquisa em Infecção Hospitalar (LAPIH). Rio de Janeiro, RJ, Brasil.
Ontario Veterinary College (OVC), University of Guelph, Gordon St & College Ave W, 2 College Ave W, Guelph, ON N1G 2W1, Canada.
Universidade Federal Rural do Rio de Janeiro. Instituto de Veterinária. Departamento de Microbiologia e Imunologia Veterinária. Laboratório de Bacteriologia Veterinária (LABACVET). Seropédica, RJ, Brasil.
Universidade Federal Rural do Rio de Janeiro. Instituto de Veterinária. Departamento de Microbiologia e Imunologia Veterinária. Laboratório de Bacteriologia Veterinária (LABACVET). Seropédica, RJ, Brasil.
Universidade Federal Rural do Rio de Janeiro. Instituto de Veterinária. Departamento de Microbiologia e Imunologia Veterinária. Laboratório de Bacteriologia Veterinária (LABACVET). Seropédica, RJ, Brasil.
Abstract in Portuguese
Complexo Acinetobacter calcoaceticus- Acinetobacter baumannii em animais: identificação e perfil de resistência antimicrobiana.] Acinetobacter spp. está emergindo como um importante patógeno humano e veterinário, principalmente devido à resistência intrínseca e adquirida aos antimicrobianos. Apesar de sua relevância.
Abstract
Acinetobacter spp. is emerging as an important human and veterinary pathogen, mostly due to intrinsic and acquired resistance to antimicrobials. Despite its public health relevance, little is known about the prevalence, role of different Acinetobacter species and antimicrobial resistance profile of animal-origin isolates. Traditional phenotypic tests may fail to discriminate Acinetobacter species, therefore molecular analyses are often required as a complementary approach. The objectives of this study were to evaluate the occurrence of strains of the Acinetobacter calcoaceticus-Acinetobacter baumannii (Acb) complex isolated from animal infections including urinary tract infections, otitis, piodermitis and pododermatitis, and its resistance profile against different antimicrobial classes, including carbapenems. All Gram-negative coccobacilli isolates were characterized by MALDI-TOF and multiplex PCR, and the disk diffusion test was used to investigate multi-drug resistance (MDR) and carbapenem resistance genes by PCR as preconized by the standard guidelines. MALDI-TOF technique identified 21 strains belonging to the Acb complex (10 A. pittii, 8 A. baumannii, 3 A. nosocomialis, 1 A. ursingii, and 1 A. venetianus). Multiplex PCR confirmed the results of MALDI-TOF for 20 strains. Eight strains (34.78%) were classified as MDR, being 50% (4/8) A. baumannii, 37.5% (3/8) A. pittii, and 12.5% (1/8) A. nosocomialis. None of the isolates presented phenotypic carbapenemase production. Considering the carbapenem resistance genes, 26.09% (6/23) of the isolates presented one or more carbapenemase genes. From these, 50% (3/6) presented only blaVIM, 33.33% (2/6) presented only blaIMP, and 16.67% (1/6) presented blaIMP e blaVIM, simultaneously. These genes were detected among A. pittii isolates mostly (66.67%, 4/6). This study provides further insights into the occurrence and resistance profile of Acinetobacter of animal origin.
Keywords in Portuguese
Complexo de Acinetobacter
Produção de carbapenemases
Resistência antimicrobiana
 
Keywords
Acinetobacter complex
Carbapenemase production
Antimicrobial resistance
 
Publisher
Colégio Brasileiro de Patologia Animal - CBPA
Citation
HOLMSTRöM, Thérèsse C. H. et al. Acinetobacter calcoaceticus-Acinetobacter baumannii complex in animals: identification and antimicrobial resistance profile. Pesquisa Veterinária Brasileira, v. 42, e07043, p. 1 - 7, 2022.
DOI
10.1590/1678-5150-PVB-7043
ISSN
1678-5150