Author
Affilliation
Abstract
Antimicrobial resistance (AMR) is a major threat to global health. Understanding the emergence, evolution, and transmission of individual antibiotic
resistance genes (ARGs) is essential to develop sustainable strategies combatting this threat. Here, we use metagenomic sequencing to analyse ARGs in
757 sewage samples from 243 cities in 101 countries, collected from 2016 to
2019. We find regional patterns in resistomes, and these differ between subsets
corresponding to drug classes and are partly driven by taxonomic variation.
The genetic environments of 49 common ARGs are highly diverse, with most
common ARGs carried by multiple distinct genomic contexts globally and
sometimes on plasmids. Analysis of flanking sequence revealed ARG-specific
patterns of dispersal limitation and global transmission. Our data furthermore
suggest certain geographies are more prone to transmission events and
should receive additional attention.
Publisher
Nature Communications
Citation
MUNK, Patrick et al. Genomic analysis of sewage from 101 countries reveals global landscape of antimicrobial resistance. Nature Communications, v. 13, 7251, p; 1- 16, Dec. 2022.
DOI
10.1038/s41467-022-34312-7
ISSN
2041-1723
Notes
Patrick Munk, Christian Brinch, Frederik Duus Møller, Thomas N. Petersen, Rene S. Hendriksen, Anne Mette Seyfarth, Jette S. Kjeldgaard, Christina Aaby Svendsen, Bram van Bunnik, Fanny Berglund, D. G. Joakim Larsson, Marion Koopmans, Mark Woolhouse, Frank M. Aarestrup, Artan Bego, Pablo Power, Catherine Rees, Dionisia Lambrinidis, Elizabeth Heather Jakobsen Neilson, Karen Gibb, Kris Coventry, Peter Collignon, Susan Cassar, Franz Allerberger, Anowara Begum, Zenat Zebin Hossain, Carlon Worrell, Olivier Vandenberg, Ilse Pieters, Dougnon Tamègnon Victorien, Angela Daniela Salazar Gutierrez, Freddy Soria, Vesna Rudić Grujić, Nataša Mazalica, Teddie O. Rahube, Carlos Alberto Tagliati, Dalia Rodrigues, Guilherme Oliveira, Larissa Camila Ribeiro de Souza, Ivan Ivanov, Bonkoungou Isidore Juste, Traoré Oumar, Thet Sopheak, Yith Vuthy, Antoinette Ngandjio, Ariane Nzouankeu, Ziem A. Abah Jacques Olivier, Christopher K. Yost, Pratik Kumar, Satinder Kaur Brar, Djim-Adjim Tabo, Aiko D. Adell, Esteban Paredes-Osses, Maria Cristina Martinez, Sara Cuadros-Orellana, Changwen Ke, Huanying Zheng, Li Baisheng, Lok Ting Lau, Teresa Chung, Xiaoyang Jiao, Yongjie Yu, Zhao JiaYong, Johan F. Bernal Morales, Maria Fernanda Valencia, Pilar Donado-Godoy, Kalpy Julien Coulibaly, Jasna Hrenovic, Matijana Jergović, Renáta Karpíšková, Zozo Nyarukweba Deogratias, Bodil Elsborg, Lisbeth Truelstrup Hansen, Pernille Erland Jensen, Mohamed Abouelnaga, Mohamed Fathy Salem, Marliin Koolmeister, Mengistu Legesse, Tadesse Eguale, Annamari Heikinheimo, Soizick Le Guyader, Julien Schaeffer, Jose Eduardo Villacis, Bakary Sanneh, Lile Malania, Andreas Nitsche, Annika Brinkmann, Sara Schubert, Sina Hesse, Thomas U. Berendonk, Courage Kosi Setsoafia Saba, Jibril Mohammed, Patrick Kwame Feglo, Regina Ama Banu, Charalampos Kotzamanidis, Efthymios Lytras, Sergio A. Lickes, Bela Kocsis, Norbert Solymosi, Thorunn R. Thorsteinsdottir, Abdulla Mohamed Hatha, Mamatha Ballal, Sohan Rodney Bangera, Fereshteh Fani, Masoud Alebouyeh, Dearbhaile Morris, Louise O’Connor, Martin Cormican, Jacob Moran-Gilad, Antonio Battisti, Elena Lavinia Diaconu, Gianluca Corno, Andrea Di Cesare, Patricia Alba, Junzo Hisatsune, Liansheng Yu, Makoto Kuroda, Motoyuki Sugai, Shizuo Kayama, Zeinegul Shakenova, Ciira Kiiyukia, Eric Ng’eno, Lul Raka, Kazi Jamil, Saja Adel Fakhraldeen, Tareq Alaati, Aivars Bērziņš, Jeļena Avsejenko, Kristina Kokina, Madara Streikisa, Vadims Bartkevics, Ghassan M. Matar, Ziad Daoud, Asta Pereckienė, Ceslova Butrimaite-Ambrozeviciene, Christian Penny, Alexandra Bastaraud, Tiavina Rasolofoarison, Jean-Marc Collard, Luc Hervé Samison, Mala Rakoto Andrianarivelo, Daniel Lawadi Banda, Arshana Amin, Heraa Rajandas, Sivachandran Parimannan, David Spiteri, Malcolm Vella Haber, Sunita J. Santchurn, Aleksandar Vujacic, Dijana Djurovic, Brahim Bouchrif, Bouchra Karraouan, Delfino Carlos Vubil, Pushkar Pal, Heike Schmitt, Mark van Passel, Gert-Jan Jeunen, Neil Gemmell, Stephen T. Chambers, Fania Perez Mendoza, Jorge Huete-Pιrez, Samuel Vilchez, Akeem Olayiwola Ahmed, Ibrahim Raufu Adisa, Ismail Ayoade Odetokun, Kayode Fashae, Anne-Marie Sørgaard, Astrid Louise Wester, Pia Ryrfors, Rune Holmstad, Mashkoor Mohsin, Rumina Hasan, Sadia Shakoor, Natalie Weiler Gustafson, Claudia Huber Schill, Maria Luz Zamudio Rojas, Jorge Echevarria Velasquez, Bonifacio B. Magtibay, Kris Catangcatang, Ruby Sibulo, Felipe Campos Yauce, Dariusz Wasyl, Celia Manaia, Jaqueline Rocha, Jose Martins, Pedro Álvaro, Doris Di Yoong Wen, Hanseob Shin, Hor-Gil Hur, Sukhwan Yoon, Golubinka Bosevska, Mihail Kochubovski, Radu Cojocaru, Olga Burduniuc, Pei-Ying Hong, Meghan Rose Perry, Amy Gassama, Vladimir Radosavljevic, Moon Y. F. Tay, Rogelio Zuniga-Montanez, Stefan Wuertz, Dagmar Gavačová, Katarína Pastuchová, Peter Truska, Marija Trkov, Karen Keddy, Kerneels Esterhuyse, Min Joon Song, Marcos Quintela-Baluja, Mariano Gomez Lopez, Marta Cerdà-Cuéllar, R. R. D. P. Perera, N. K. B. K. R. G. W. Bandara, H. I. Premasiri, Sujatha Pathirage, Kareem Charlemagne, Carolin Rutgersson, Leif Norrgren, Stefan Örn, Renate Boss, Tanja Van der Heijden, Yu-Ping Hong, Happiness Houka Kumburu, Robinson Hammerthon Mdegela, Yaovi Mahuton Gildas Hounmanou, Kaknokrat Chonsin, Orasa Suthienkul, Visanu Thamlikitkul, Ana Maria de Roda Husman, Bawimodom Bidjada, Berthe-Marie Njanpop-Lafourcade, Somtinda Christelle Nikiema-Pessinaba, Belkis Levent, Cemil Kurekci, Francis Ejobi, John Bosco Kalule, Jens Thomsen, Ouidiane Obaidi, Laila Mohamed Jassim, Andrew Moore, Anne Leonard, David W. Graham, Joshua T. Bunce, Lihong Zhang, William H. Gaze, Brett Lefor, Drew Capone, Emanuele Sozzi, Joe Brown, John Scott Meschke, Mark D. Sobsey, Michael Davis, Nicola Koren Beck, Pardi Sukapanpatharam, Phuong Truong, Ronald Lilienthal, Sanghoon Kang, Thomas E. Wittum, Natalia Rigamonti, Patricia Baklayan, Chinh Dang Van, Doan Minh Nguyen Tran, Nguyen Do Phuc & Geoffrey Kwenda.
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