Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/5711
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dc.contributor.authorAndrade, Sonia Gumes
dc.contributor.authorGuerret, Sylviane Stocker
dc.contributor.authorPimentel, Ariane R
dc.contributor.authorGrimaud, Jean Alexis
dc.date.accessioned2012-10-22T20:37:09Z
dc.date.available2012-10-22T20:37:09Z
dc.date.issued1991
dc.identifier.citationANDRADE, S.G. et al. Reversibility of cardiac fibrosis in mice chronically infected with Trypanosoma cruzi, under specific chemotherapy. Memórias do Instituto Oswaldo Cruz, v. 86, n. 2, p. 187-200, apr.-jun. 1991.
dc.identifier.issn0074-0276
dc.identifier.urihttps://www.arca.fiocruz.br/handle/icict/5711
dc.language.isoeng
dc.rightsopen access
dc.titleReversibility of cardiac fibrosis in mice chronically infected with Trypanosoma cruzi, under specific chemotherapy.
dc.typeArticle
dc.description.abstractenThis investigation was performed to verify the effect of specific chemotherapy (Benznidazole or MK-436) on the inflammatory and fibrotic cardiac alterations in mice chronically infected with the strains 21 SF (Type II) and Colombian (Type III) of Trypanosoma cruzi. To obtain chronically infected mice, two groups of 100 Swiss mice each, were infected with either the 21 SF or the Colombian strain (2 x 10(4) and 5 x 10(4) blood forms respectively). The rate of mortality in the acute phase was of 80% for both groups. Twenty surviving mice chronically infected with the 21 SF strain and 20 with the Colombian strain were then divided in treated and untreated groups. Excluding those that died during the course of treatment, 14 mice chronically infected with the 21 SF strain and 15 with the Colombian strain were finally evaluated in the present study. Chemotherapy was performed with Benznidazole (N-benzil-2-nitro-1-imidazolacetamide) in the dose of 100 mg/k.b.w/day, for 60 days, or with the MK-436 (3(1-methyl-5 nitroimidazol-2-yl) in two daily doses of 250 mg/k.b.w, for 20 days. Parasitological cure tests were performed (xenodiagnosis, haemoculture, subinoculation of the blood into newborn mice), and serological indirect immunofluorescence test. The treated and untreated mice as well as intact controls were killed at different periods after treatment and the heart were submitted to histopathological study with hematoxilineosin and picrosirius staining; ultrastructural study; collagen immunotyping, fibronectin and laminin identification by immunofluorescence tests. Results: the untreated controls either infected with 21 SF or Colombian strain, showed inflammatory and fibrotic alterations that were mild to moderate with the 21 SF strain and intense with the Colombian strain. Redpicrosirius staining showed bundles of collagen in the interstitial space and around cardiac fibers. Increased deposits of matritial components and collagen fibers, macrophages and fibroblasts appeared at the ultrastructural examination. Deposits of fibronectin, laminin, pro-III and IV collagens were seen, most intense in those infected with the Colombian strain. Treated mice, parasitologically cured, presented clear-cut regression of the inflammatory lesions and of the interstitial matrix thickening. Mice infected with the Colombian strain and treated with MK-436, was parasitologically cured in 5/6 cases and showed mild inflammatory infiltration and fibrosis. The mice treated with Benznidazole (Colombian strain) did not cure and showed moderate fibrosis and inflammation. Treatment of the mice infected with the 21 SF with Benznidazole determined parasitological cure of all animals, that showed mild inflammation and fibrosis of the myocardium
dc.creator.affilliationFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil
dc.creator.affilliationInstitut Pasteur de Lyon. Centro de Microscopie Eletronique. Lyon, France
dc.creator.affilliationFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil
dc.creator.affilliationInstitut Pasteur de Lyon. Centro de Microscopie Eletronique. Lyon, France
dc.subject.enTrypanosoma cruzi
dc.subject.enChronic myocarditis
dc.subject.enCollagen immunotyping
dc.subject.enFibronectin
dc.subject.enLaminin
dc.subject.enChemotherapy
dc.subject.decsCardiomiopatia Chagásica/quimioterapia
dc.subject.decsNitroimidazóis/uso terapêutico
dc.subject.decsAnimais
dc.subject.decsCardiomiopatia Chagásica/parasitologia
dc.subject.decsCardiomiopatia Chagásica/patologia
dc.subject.decsModelos Animais de Doenças
dc.subject.decsFibrose Endomiocárdica/quimioterapia
dc.subject.decsFibrose Endomiocárdica/parasitologia
dc.subject.decsFibrose Endomiocárdica/patologia
dc.subject.decsMatriz Extracelular/ultraestrutura
dc.subject.decsCamundongos
dc.subject.decsMiocárdio/ultraestrutura
dc.subject.decsEspecificidade da Espécie
Appears in Collections:BA - IGM - Artigos de Periódicos

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