Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/5846
Title: Detection and quantification of Leptospira interrogans in hamster and rat kidney samples: immunofluorescent imprints versus real-time PCR
Authors: Chagas Júnior, Adenizar Delgado das
Silva, Caroline Luane Rabelo da
Soares, Luciane Marieta
Santos, Cleiton Silva
Silva, Carlos D. C. M
Athanazio, Daniel Abensur
Reis, Mitermayer Galvão dos
McBride, Flávia Weykamp da Cruz
McBride, Alan John Alexander
Affilliation: Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, Bahia, Brasil
Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, Bahia, Brasil
Federal University of Bahia. Health Sciences Institute. Salvador, Bahia, Brasil
Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, Bahia, Brasil
Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, Bahia, Brasil
Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, Bahia, Brasil
Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, Bahia, Brasil
Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, Bahia, Brasil
Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, Bahia, Brasil
Abstract: A major limitation in the clinical management and experimental research of leptospirosis is the poor performance of the available methods for the direct detection of leptospires. In this study, we compared real-time PCR (qPCR), targeting the lipL32 gene, with the immunofluorescent imprint method (IM) for the detection and quantification of leptospires in kidney samples from the rat and hamster experimental models of leptospirosis. Using a virulent strain of Leptospira interrogans serovar Copenhageni, a chronic infection was established in the rat model, which were euthanized 28 days post-infection, while the hamster model simulated an acute infection and the hamsters were euthanized eight days after inoculation. Leptospires in the kidney samples were detected using culture isolation, qPCR and the IM, and quantified using qPCR and the IM. In both the acute and chronic infection models, the correlation between quantification by qPCR and the IM was found to be positive and statistically significant (P<0.05). Therefore, this study demonstrates that the IM is a viable alternative for not only the detection but also the quantification of leptospires, particularly when the use of qPCR is not feasible.
DeCS: Rim/metabolismo
Leptospira interrogans/metabolismo
Leptospirose/microbiologia
Microscopia de Fluorescência/métodos
Reação em Cadeia da Polimerase em Tempo Real/métodos
Animais
Cricetinae
Feminino
Infecção
Leptospirose/patologia
Mesocricetus
Ratos
Ratos Wistar
Análise de Sequência de DNA
Issue Date: 2012
Citation: CHAGAS JUNIOR, A.D. et al. Detection and Quantification of Leptospira interrogans in Hamster and and rat kidney samples: immunofluorescent imprints versus real-time PCR. PLoS One, v. 7, n. 2, p. e32712, 2012.
ISSN: 1932-6203
10.1371/journal.pone.0032712
Appears in Collections:IGM - Artigos de Periódicos

Files in This Item:
File Description SizeFormat 
Chagas Junior AD Detection and quantification....pdf173.91 kBAdobe PDFThumbnail
View/Open


FacebookTwitterDeliciousLinkedInGoogle BookmarksBibTex Format mendeley Endnote DiggMySpace

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.