| Author | Albuquerque, Priscila C. | |
| Author | Fonseca, Fernanda L. | |
| Author | Dutra, Fabianno F. | |
| Author | Bozza, Marcelo T. | |
| Author | Frases, Susana | |
| Author | Casadevall, Arturo | |
| Author | Rodrigues, Marcio L. | |
| Access date | 2023-07-18T04:21:14Z | |
| Available date | 2023-07-18T04:21:14Z | |
| Document date | 2014 | |
| Citation | ALBUQUERQUE, Priscila C. et al. Cryptococcus neoformans glucuronoxylomannan fractions of different molecular masses are functionally distinct. Future Microbiology, v. 9, n. 2, p. 147-161, 2014. | en_US |
| ISSN | 1746-0913 | en_US |
| URI | https://www.arca.fiocruz.br/handle/icict/59631 | |
| Language | eng | en_US |
| Publisher | Future Medicine | en_US |
| Rights | open access | en_US |
| MeSH | Animals | en_US |
| MeSH | Cryptococcosis | en_US |
| MeSH | Cryptococcus neoformans | en_US |
| MeSH | Cytokines | en_US |
| MeSH | Fungal Capsules | en_US |
| MeSH | Macrophages | en_US |
| MeSH | Mice | en_US |
| MeSH | Mice, Inbred C57BL | en_US |
| MeSH | Mice, Knockout | en_US |
| MeSH | Molecular Weight | en_US |
| MeSH | Polysaccharides | en_US |
| MeSH | Protein Binding | en_US |
| MeSH | Subcellular Fractions | en_US |
| Title | Cryptococcus neoformans glucuronoxylomannan fractions of different molecular masses are functionally distinct | en_US |
| Type | Article | en_US |
| DOI | 10.2217/FMB.13.163 | |
| Abstract | Aims: Glucuronoxylomannan (GXM) is the major polysaccharide component of Cryptococcus neoformans. We evaluated in this study whether GXM fractions of different molecular masses were functionally distinct. Materials & methods: GXM samples isolated from C. neoformans cultures were fractionated to generate polysaccharide preparations differing in molecular mass. These fractions were used in experiments focused on the association of GXM with cell wall components of C. neoformans, as well as on the interaction of the polysaccharide with host cells. Results & conclusion: GXM fractions of variable molecular masses bound to the surface of a C. neoformans acapsular mutant in a punctate pattern that is in contrast to the usual annular pattern of surface coating observed when GXM samples containing the full molecular mass range were used. The polysaccharide samples were also significantly different in their ability to stimulate cytokine production by host cells. Our findings indicate that GXM fractions are functionally distinct depending on their mass. | en_US |
| Affilliation | Fundação Oswaldo Cruz. Centro de Desenvolvimento Tecnológico em Saúde. Rio de Janeiro, RJ, Brazil / Universidade Federal do Rio de Janeiro. Instituto de Microbiologia Paulo de Gões. Rio de Janeiro, RJ, Brazil. | en_US |
| Affilliation | Fundação Oswaldo Cruz. Centro de Desenvolvimento Tecnológico em Saúde. Rio de Janeiro, RJ, Brazil / Universidade Federal do Rio de Janeiro. Instituto de Microbiologia Paulo de Gões. Rio de Janeiro, RJ, Brazil. | en_US |
| Affilliation | Universidade Federal do Rio de Janeiro. Instituto de Microbiologia Paulo de Gões. Rio de Janeiro, RJ, Brazil. | en_US |
| Affilliation | Universidade Federal do Rio de Janeiro. Instituto de Microbiologia Paulo de Gões. Rio de Janeiro, RJ, Brazil. | en_US |
| Affilliation | Universidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho. Rio de Janeiro, RJ, Brazil. | en_US |
| Affilliation | Fundação Oswaldo Cruz. Centro de Desenvolvimento Tecnológico em Saúde. Rio de Janeiro, RJ, Brazil / Universidade Federal do Rio de Janeiro. Instituto de Microbiologia Paulo de Gões. Rio de Janeiro, RJ, Brazil. | en_US |
| Affilliation | Fundação Oswaldo Cruz. Centro de Desenvolvimento Tecnológico em Saúde. Rio de Janeiro, RJ, Brazil / Universidade Federal do Rio de Janeiro. Instituto de Microbiologia Paulo de Gões. Rio de Janeiro, RJ, Brazil. | en_US |
| Subject | Cryptococcus neoformans | en_US |
| Subject | Glucuronoxylomannan | en_US |
| Subject | Host response | en_US |
| Subject | Molecular mass | en_US |
| Subject | Polysaccharides | en_US |
| e-ISSN | 1746-0921 | |
