| Author | Nunes, Sara | |
| Author | Tibúrcio, Rafael | |
| Author | Silva, Icaro Bonyek | |
| Author | Oliveira, Pablo Rafael | |
| Author | Khouri, Ricardo | |
| Author | Boaventura, Viviane | |
| Author | Barral, Aldina | |
| Author | Brodskyn, Cláudia | |
| Author | Tavares, Natalia Machado | |
| Access date | 2023-11-09T19:43:57Z | |
| Available date | 2023-11-09T19:43:57Z | |
| Document date | 2023 | |
| Citation | NUNES, Sara Nunes et al. Transcriptome analysis identifies the crosstalk between dendritic and natural killer cells in human cutaneous leishmaniasis. Microorganisms, v. 11, n. 8, p. 1-14, 2023. | en_US |
| ISSN | 2076-2607 | en_US |
| URI | https://www.arca.fiocruz.br/handle/icict/61265 | |
| Sponsorship | Fundação de Amparo à Pesquisa do Estado da Bahia (FAPESB).
Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq).
(Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES ). | en_US |
| Language | eng | en_US |
| Publisher | MDPI | en_US |
| Rights | open access | en_US |
| Subject in Portuguese | Leishmania braziliensis | en_US |
| Subject in Portuguese | Células assassinas naturais | en_US |
| Subject in Portuguese | Células dendríticas | en_US |
| Subject in Portuguese | Transcriptoma | en_US |
| Subject in Portuguese | Leishmaniose cutânea | en_US |
| Title | Transcriptome analysis identifies the crosstalk between dendritic and natural killer cells in human cutaneous leishmaniasis | en_US |
| Type | Article | en_US |
| DOI | 10.3390/microorganisms11081937 | |
| Abstract | Skin ulcers of cutaneous leishmaniasis (CL) are characterized by a localized inflammatory response mediated by innate and adaptive immune cells, including dendritic cells (DC) and natural killer (NK) cells. Bidirectional interactions between DCs and NK cells contribute to tailor leishmaniasis outcome. Despite advances in the Leishmania biology field in recent decades, the mechanisms involved in DC/NK-mediated control of Leishmania sp. pathogenesis as well as the cellular and molecular players involved in such interaction remain unclear. The present study sought to investigate canonical pathways associated with CL arising from Leishmania braziliensis infection. Initially, two publicly available microarray datasets of skin biopsies from active CL lesions were analyzed, and five pathways were identified using differentially expressed genes. The “Crosstalk between DCs and NK cells” pathway was notable due to a high number of modulated genes. The molecules significantly involved in this pathway were identified, and our findings were validated in newly obtained CL biopsies. We found increased expression of TLR4, TNFRSF1B, IL-15, IL-6, CD40, CCR7, TNF and IFNG, confirming the analysis of publicly available datasets. These findings reveal the “crosstalk between DCs and NK cells” as a potential pathway to be further explored in the pathogenesis of CL, especially the expression of CCR7, which is correlated with lesion development. | en_US |
| Affilliation | Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Laboratório de Interação Parasita-Hospedeiro e Epidemiologia. Salvador, BA, Brasil. | en_US |
| Affilliation | Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Laboratório de Interação Parasita-Hospedeiro e Epidemiologia. Salvador, BA, Brasil. | en_US |
| Affilliation | Instituto Federal Baiano. Xique-Xique, BA, Brasil. | en_US |
| Affilliation | Universidade Federal da Bahia. Instituto de Biologia. Salvador, BA, Brasil. | en_US |
| Affilliation | Universidade Federal da Bahia. Instituto de Biologia. Salvador, BA, Brasil / Fundação Oswaldo Cruz. Instituto Gonçalo Moniz, Laboratório de Doenças Infecciosas Transmitidas por Vetores. Salvador, BA, Brasil. | en_US |
| Affilliation | Universidade Federal da Bahia. Instituto de Biologia. Salvador, BA, Brasil / Fundação Oswaldo Cruz. Instituto Gonçalo Moniz, Laboratório de Doenças Infecciosas Transmitidas por Vetores. Salvador, BA, Brasil. | en_US |
| Affilliation | Universidade Federal da Bahia. Instituto de Biologia. Salvador, BA, Brasil / Fundação Oswaldo Cruz. Instituto Gonçalo Moniz, Laboratório de Doenças Infecciosas Transmitidas por Vetores. Salvador, BA, Brasil. | en_US |
| Affilliation | Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Laboratório de Interação Parasita-Hospedeiro e Epidemiologia. Salvador, BA, Brasil / Universidade Federal da Bahia. Instituto de Biologia. Salvador, BA, Brasil. | en_US |
| Affilliation | Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Laboratório de Interação Parasita-Hospedeiro e Epidemiologia. Salvador, BA, Brasil / Universidade Federal da Bahia. Instituto de Biologia. Salvador, BA, Brasil. | en_US |
| Subject | Leishmania braziliensis | en_US |
| Subject | Natural killer cells | en_US |
| Subject | Dendritic cells | en_US |
| Subject | Transcriptome | en_US |
| Subject | Cutaneous leishmaniasis | en_US |
| DeCS | Leishmania braziliensis | en_US |
| DeCS | Células Matadoras Naturais | en_US |
| DeCS | Células Dendríticas | en_US |
| DeCS | Transcriptoma | en_US |
| DeCS | Leishmaniose | en_US |
| DeCS | Infecções | en_US |
