Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/6197
Title: Schizodeme analysis of Trypanosoma cruzi Colombian strain clones isolated from the acute phase of murine infection
Authors: Camandaroba, Edson Luiz Paes
Reis, Eliana Almeida Gomes
Reis, Mitermayer Galvão dos
Andrade, Sonia Gumes
Affilliation: Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, Bahia, Brasil / Universidade Estadual de Feira de Santana. Feira de Santana, BA, Brasil
Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, Bahia, Brasil
Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, Bahia, Brasil
Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, Bahia, Brasil
Abstract: Colombian strain of Trypanosoma cruzi, biodeme Type III (T. cruzi I), has been cloned by micromanipulation at two phases of the acute infection: early (10 days ) and advanced (30 days). Twelve clones were obtained therefrom. Characterization by their biological and biochemical behavior showed an identity among the several clones and their parental strain, albeit with different degrees of virulence. Molecular characterization of the kinetoplast DNA (kDNA) after amplification by polymerase chain reaction revealed identical profiles of the bands from the kDNA minicircle by the analysis of restriction fragment lenght polymorphism for the isolated clones, their parental strain, and to the clones isolated at two different phases of the infection. Results suggest the predominance of a "principal clone", in the composition of the Colombian strain, responsible for the biological and biochemical behavior. However, no relationship was detected between the molecular profile of kDNA and the degree of virulence presented by the several clones.
Keywords: Trypanosoma cruzi
Colombian strain clones
Schizodeme
DeCS: DNA de Cinetoplasto/genética
Trypanosoma cruzi/genética
Doença Aguda
Doença de Chagas/parasitologia
Células Clonais
Colômbia
Reação em Cadeia da Polimerase
Polimorfismo de Fragmento de Restrição
Trypanosoma cruzi/enzimologia
Trypanosoma cruzi/patogenicidade
Virulência
Animais
Camundongos
Issue Date: 2006
Citation: CAMANDAROBA, E. L. P. et al. Schizodeme analysis of Trypanosoma cruzi Colombian strain clones isolated from the acute phase of murine infection. Memórias do Instituto Oswaldo Cruz, v. 101, n. 6, p. 613-615, sept. 2006.
ISSN: 0074-0276
Copyright: open access
Appears in Collections:BA - IGM - Artigos de Periódicos

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