Protein profile analysis by SDS-PAGE of Mycoplasma gallisepticum strains S6(208) and F-K810 grown in Hayflick's and Frey's media

Universidade Federal Rural do Rio de Janeiro. Instituto de Veterinária. Seropédica, RJ, Brasil.
Universidade Federal Rural do Rio de Janeiro. Instituto de Veterinária. Seropédica, RJ, Brasil.
Universidade Federal Rural do Rio de Janeiro. Instituto de Veterinária. Seropédica, RJ, Brasil.
Universidade Federal Rural do Rio de Janeiro. Instituto de Veterinária. Seropédica, RJ, Brasil.
Universidade Federal Fluminense. Faculdade de Veterinária. Niterói, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto de Tecnologia em Imunobiológicos (Bio-Manguinhos). Rio de Janeiro, RJ, Brasil.

Abstract

The Mycoplasma gallisepticum strains [wild-type S6 (208) and a vaccine-type F-K810] grown in Frey´s and Hayflick´s media were analyzed by SDS-PAGE. No visual changes in the protein profiles of these strains were observed regardless of the media composition used, although the polyacrylamide gel electrophoretograms showed minor differences do exist when densitometer traces of the gel are compared. Both strains were easily differentiated on SDS-PAGE analysis by a peptide band p75, that is specific for MG F-K810 strain, used as vaccine.

Keywords

Mycoplasma gallisepticum
SDS-PAGE
Protein profile

Publisher

Sociedade Brasileira de Microbiologia

Citation

FERRAZ, Priscila N. et al. Protein profile analysis by SDS-PAGE of Mycoplasma gallisepticum strains S6(208) and F-K810 grown in Hayflick's and Frey's media. Brazilian Journal of Microbiology, v. 31, n. 2, p. 113-115, Jun. 2000.

DOI

10.1590/S1517-83822000000200010

ISSN

15178382

Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/64160

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Open access

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