Shrimp allergy is a significant concern due to its high allergenicity and association with anaphylactic reactions. However, the current understanding of this condition is limited, and novel therapeutic strategies are required. In this study, we aimed to establish a new experimental model of shrimp allergy to facilitate the evaluation of prophylactic and therapeutic approaches. BALB/c mice were subcutaneously sensitized with 100 μg of Litopenaeus vannamei shrimp proteins adsorbed in 1 mg of aluminum hydroxide on day 0, followed by a booster (100 μg of shrimp proteins only) on day 14. For oral challenge, 5 mg/ml of shrimp proteins were administered in the water from day 21 to day 35. The shrimp extract analysis revealed the presence of at least 4 major allergens reported in L. vannamei. Allergic mice exhibited a significant increase in IL-4 and IL-10 production in restimulated cervical draining lymph node cells following sensitization. High levels of serum anti-shrimp IgE and IgG1 antibodies indicated the development of shrimp allergies, and the Passive Cutaneous Anaphylaxis assay confirmed an IgE-mediated response. Immunoblotting analysis further demonstrated the presence of antibodies against multiple shrimp extract antigens. These findings were corroborated by detecting anti-shrimp IgA production in intestinal lavage samples and morphometric changes in the intestinal mucosa. Thus, this new experimental protocol provides a valuable tool for the assessment of prophylactic and therapeutic approaches in shrimp allergy research for potential clinical applications at an international level in the field of immunology.