Mycobacterium tuberculosis (Mtb), the causative agent of tuberculosis (TB), remains one of the main challenges to public health with were 1.6 million deaths and 10.6 million new cases in 2021. Bacillus Calmette-Guérrin (BCG) is the only licensed vaccine against TB, despite its variable efficacy (0-80%) against the pulmonary from of the disease in adults. Recent clinical trials indicate the importance of exploring diverse classes of antigens to increase the vaccine protection already induced by BCG. Thus, lipid antigens are promising, considering their high concentration in the mycobacterial cell wall and central role in host-pathogen interactions. Here, the levels of IFN-γ and IL-10 induced by nonpolar lipids of BCG Moreau and Mtb in the supernatant of peripheral blood mononuclear cells (PBMC) cultures from healthy individuals were compared. Nonpolar lipid extracts were obtained from planktonic cultures with the addition of petroleum ether and methanol with 0.3% NaCl (1:10) for cell culture plates sensitization. Culture supernatants were collected to measure IFN-γ and IL-10 levels by ELISA after 24_h, 48_h and 72_h of culture. Mtb nonpolar lipid extract induced higher concentrations of IFN-γ and IL-10, unlike BCG Moreau’s lipid extract. After 24_h and 48_h, Mtb lipid extract induced a higher concentration of IFN-γ, when compared to BCG Moreau (p<0.05) and unmarked control (NCS) (p<0.001). Furthermore, nonpolar lipids from Mtb induced greater productions of IL-10 in all times-points of cultures evaluated, when compared to NCS (p<0.05 and p<0.0001). BCG’s lipid extract induced only an increase of IL- 10 levels after 72_h (p<0.0001). The further characterization of the cellular response induced by mycobacterial nonpolar lipids extracts might facilitate future identification of isolated lipids with greater antigenic potential that could be considered as adjuvants in new vaccine schemes against TB.