| Author | Cardoso, Thayná Laner | |
| Author | Freitas, Stella Buchhorn de | |
| Author | Seixas Neto, Amilton Clair Pinto | |
| Author | Balassiano, Ilana Teruszkin | |
| Author | Hartwig, Daiane Drawanz | |
| Access date | 2024-09-26T12:54:09Z | |
| Available date | 2024-09-26T12:54:09Z | |
| Document date | 2024 | |
| Citation | CARDOSO, Thayná Laner et al. Advancing serologic diagnosis: assessing the efficacy of rErpY-like protein in human leptospirosis detection. Brazilian Journal of Microbiology, v. 55, p. 2279-2284, 28 May 2024. | |
| ISSN | 1517-8382 | |
| URI | https://www.arca.fiocruz.br/handle/icict/66117 | |
| Description | Produção científica do Laboratório de Zoonoses Bacterianas. | pt_BR |
| Sponsorship | This work was financed in part by the Coordenação de Aperfeiçoamento de Pessoal de Nível Superior - Brasil (CAPES, http://www.capes.gov.br/) - Finance Code 001, Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq, http://www.cnpq.br/)– Process Code 425107/2018-3 and 307895/2020-3 and Fundação de Amparo à Pesquisa do Estado do Rio Grande do Sul (FAPERGS, https://fapergs.rs.gov.br/inicial)– Process Code 21/2551-0001941-7. | |
| Language | eng | en_US |
| Publisher | Springer | |
| Rights | restricted access | |
| Title | Advancing serologic diagnosis: assessing the efficacy of rErpY-like protein in human leptospirosis detection | en_US |
| Type | Article | |
| DOI | 10.1007/s42770-024-01364-4 | |
| Abstract | Leptospirosis is a globally distributed infectious disease caused by pathogenic spirochetes of the Leptospira genus, often overlooked. It is estimated that the disease affects approximately one million people annually, resulting in more than 58,900 deaths. The gold standard for serodiagnosis of leptospirosis is the Microscopic Agglutination Test (MAT). However, the limitations of this technique necessitate the exploration of alternative diagnostic methods. In this study, we evaluated the ErpY-like recombinant protein (rErpY-like) in the development of a serologic diagnostic assay for human leptospirosis. Eighty-six human sera samples, characterized by MAT, underwent evaluation through indirect IgM-ELISA and IgG-ELISA. The sensitivity and specificity values obtained from IgM-ELISA were 60% and 76%, respectively, while those from IgG-ELISA were 96.4% and 100%, respectively. The use of the rErpY-like protein in both IgM-ELISA and IgG-ELISA proves to be a sensitive and specific method for antibody detection. This could potentially serve as a valuable alternative tool in the diagnosis of human leptospirosis. | en_US |
| Affilliation | Federal University of Pelotas. Institute of Biology. Department of Microbiology and Parasitology. Laboratory of Bacteriology and Bioassays. Pelotas, RS, Brasil. | |
| Affilliation | Federal University of Pelotas. Institute of Biology. Department of Microbiology and Parasitology. Laboratory of Bacteriology and Bioassays. Pelotas, RS, Brasil. | |
| Affilliation | Federal University of Pelotas. Institute of Biology. Department of Microbiology and Parasitology. Laboratory of Bacteriology and Bioassays. Pelotas, RS, Brasil. | |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Zoonoses Bacterianas. Rio de Janeiro, RJ, Brasil / Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Zoonoses Bacterianas. Laboratório de Referência Nacional para Leptospirose. Rio de Janeiro, RJ, Brasil / Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Vice-Diretoria de Laboratórios de Referência, Ambulatórios e Coleções Biológicas. Coleções Microbiológicas. Coleção de Leptospira. Rio de Janeiro, RJ, Brasil. | |
| Affilliation | Federal University of Pelotas. Institute of Biology. Department of Microbiology and Parasitology. Laboratory of Bacteriology and Bioassays. Pelotas, RS, Brasil / Federal University of Pelotas. University Campus. Pelotas, RS, Brasil. | |
| Subject | rErpY-like | en_US |
| Subject | Diagnosis | en_US |
| Subject | Indirect ELISA | en_US |
| Subject | Human leptospirosis | en_US |
| e-ISSN | 1678-4405 | |
