| Author | Romagnoli, Bruno Accioly Alves | |
| Author | Lucena, Aline Castro Rodrigues | |
| Author | Freire, Eden Ribeiro | |
| Author | Rocha, Isadora Filipaki Munhoz da | |
| Author | Alves, Lysangela Ronalte | |
| Author | Goldenberg, Samuel | |
| Access date | 2024-10-20T21:41:10Z | |
| Available date | 2024-10-20T21:41:10Z | |
| Document date | 2024 | |
| Citation | ROMAGNOLI, Bruno Accioly Alves et al. TcZC3HTTP, a regulatory element that contributes to cell proliferation. Microbiology Stectrum, v. 12, n. 3, p. 1-18, Mar. 2024. | en_US |
| ISSN | 2165-0497 | en_US |
| URI | https://www.arca.fiocruz.br/handle/icict/66612 | |
| Description | SUPPLEMENTAL MATERIAL: Supplemental Captions and Figuresspectrum.02880-23-s0001.docx. Captions for Tables S1 to S12; Figures S1 and S2. Tables S1 to S10spectrum.02880-23-s0002.xlsx. Additional data for RNA-seq and proteomics. Tables S11 and S12spectrum.02880-23-s0003.xlsx. Additional data for proteomics. DATA AVAILABILITY: The RNA-seq data have been deposited at the Sequence Read Archive (SRA) database under the accession number PRJNA986951. | en_US |
| Sponsorship | CNPq | en_US |
| Language | eng | en_US |
| Publisher | American Society for Microbiology | en_US |
| Rights | open access | en_US |
| Subject in Portuguese | Trypanosoma cruzi | en_US |
| Subject in Portuguese | Regulação da expressão gênica | en_US |
| Subject in Portuguese | Proliferação celular | en_US |
| Subject in Portuguese | Estresse nutricional | en_US |
| Subject in Portuguese | Proteínas de ligação ao RNA | en_US |
| Title | TcZC3HTTP, a regulatory element that contributes to Trypanosoma cruzi cell proliferation | en_US |
| Type | Article | en_US |
| DOI | 10.1128/spectrum.02880-23 | |
| Abstract | Post-transcriptional regulation of gene expression is a critical process for adapting to and surviving Trypanosoma cruzi, a parasite with a complex life cycle. RNA-binding proteins (RBPs) are key players in this regulation, forming ribonucleoprotein complexes (messenger ribonucleoproteins) and RNA granules that control transcript stability, localization, degradation, and translation modulation. Understanding the specific roles of individual RBPs is crucial for unraveling the details of this regulatory network. In this study, we generated null mutants of the TcZC3HTTP gene, a specific RBP in the Trypanosoma family characterized by a C3H zinc finger and a DNAJ domain associated with RNA and protein binding, respectively. Through cell growth assays, we demonstrated that the absence of TcZC3HTTP or the expression of an additional tagged version impacted epimastigote growth, indicating its contribution to cell proliferation. TcZC3HTTP was found to associate with mRNAs involved in cell cycle and division in epimastigotes, while in nutritionally stressed parasites it exhibited associations with mRNAs coding for other RBPs and rRNA. Furthermore, our analysis identified that TcZC3HTTP protein partners were different during normal growth conditions compared to starvation conditions, with the latter showing enrichment of ribosomal proteins and other RBPs. Therefore, this study provides insights into TcZC3HTTP’s role in the post-tran scriptional regulation of gene expression during normal growth and nutritional stress in T. cruzi, uncovering its versatile functions in different cellular contexts. | en_US |
| Affilliation | Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Regulação da Expressão Gênica. Curitiba, PR, Brasil. | en_US |
| Affilliation | Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Ciências e Tecnologias Aplicadas em Saúde. Curitiba, PR, Brasil. | en_US |
| Affilliation | Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Regulação da Expressão Gênica. Curitiba, PR, Brasil. | en_US |
| Affilliation | Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Regulação da Expressão Gênica. Curitiba, PR, Brasil. | en_US |
| Affilliation | Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Regulação de Expressão Gênica. Curitiba, PR, Brasil / University Laval. CHU de Quebec Research Center. Division of Infectious Disease and Immunity. Research Center in Infectious Diseases. Quebec, Canada. | en_US |
| Affilliation | Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Regulação da Expressão Gênica. Curitiba, PR, Brasil. | en_US |
| Subject | Trypanosoma cruzi | en_US |
| Subject | Cell proliferation | en_US |
| Subject | RNA-binding proteins | en_US |
| Subject | Nutritional stress | en_US |
| DeCS | Trypanosoma cruzi | en_US |
| DeCS | Proliferação de Células | en_US |
| DeCS | Proteínas de Ligação a RNA | en_US |
