Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/6812
Title: Chagas' disease: TCRBV9 over-representation and sequence oligoclonality in the fine specificity of T lymphocytes in target tissues of damage
Authors: Tekiel, Valeria
Oliveira, Guilherme Corrêa de
Oliveira, Rodrigo Corrêa de
Sanchez, Daniel
Cappa, Stella Maris Gonzalez
Affilliation: Universidad de Buenos Aires. Facultad de Medicina. Departamento de Microbiologıa, Parasitologıa e Inmunologıa. Buenos Aires, Argentina/Universidad Nacional de General San Martın—CONICET. Instituto de Investigaciones Biotecnologicas. Buenos Aires, Argentina
Fundação Oswaldo Cruz. Centro de Pesquisas Rene Rachou. Belo Horizonte, Brazil
Fundação Oswaldo Cruz. Centro de Pesquisas Rene Rachou. Belo Horizonte, Brazil
Universidad Nacional de General San Martın—CONICET. Instituto de Investigaciones Biotecnologicas. Buenos Aires, Argentina
Universidad de Buenos Aires. Facultad de Medicina. Departamento de Microbiologıa, Parasitologıa e Inmunologıa. Buenos Aires, Argentina
Abstract: Using the same mouse strain and two Trypanosoma cruzisub-populations (CA-I and RA) it is possible to induce pathology in different target tissues: skeletal muscle (CA-I) or sciatic nerve and spinal cord (RA). On the other hand, T cells are directly involved in tissue injury in a strain-dependent way, resembling the abnormalities of chronic Chagas’ disease. In the present work, we examined the TCRBV repertoire and the CDR3 sequence polymorphism of T cells infiltrating spinal cord, sciatic nerve and skeletal muscle in chronically infected mice. The TCRBV9 segment was systematically over-represented in the target tissues for eachT. cruzistrain: sciatic nerve and spinal cord in RA and skeletal muscle in CA-I-infected mice. The analysis of CDR3 sequence polymorphism in the same tissues showed a high proportion of identical TCRBV9 clones in RA-infected mice: 66.6% of the TCRBV9 clones found in sciatic nerve and spinal cord expressed one out of four major CDR3 rearrangements. Sequence identity was shared among clones from sciatic nerve and spinal cord, tissues that are also damaged by passive transfer of CD8 + TL. Those observations are consistent with an antigen driven T-cell expansion sequestered at the inflammation site and demonstrate – for the first time – the presence of an oligoclonal repertoire in the antigen recognition site of over-represented T cells in nervous system tissues in chronic Chagas’ disease.
Keywords: Trypanosoma cruzi
T-cell receptor
CDR3 polymorphism
Clonal expansion
Nervous system
Issue Date: 2005
Publisher: Elsevier
Citation: TEKIEL, Valeria et al. Chagas' disease: TCRBV9 over-representation and sequence oligoclonality in the fine specificity of T lymphocytes in target tissues of damage. Acta Trop., v. 94, n. 1, p. 15-24, 2005.
ISSN: 0001-706X
Copyright: open access
Appears in Collections:MG - IRR - Artigos de Periódicos

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