Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/7105
Title: The distribution of motor proteins in the muscles and flame cells of the Schistosoma mansoni miracidium and primary sporocyst
Authors: Bahia, Diana
Avelar, Lívia das Gracas Amaral
Vigorosi, F.
Oliveira, Guilherme Corrêa de
Mortara, Renato Arruda
Affilliation: Fundação Oswaldo Cruz. Centro de Pesquisas Rene Rachou. Laboratorio de Parasitologia Celular e Molecular. Belo Horizonte, MG, Brazil
Fundação Oswaldo Cruz. Centro de Pesquisas Rene Rachou. Laboratorio de Parasitologia Celular e Molecular. Belo Horizonte, MG, Brazil
Institute of Cell Biology. Monterotondo, Rome,Italy
Fundação Oswaldo Cruz. Centro de Pesquisas Rene Rachou. Laboratorio de Parasitologia Celular e Molecular. Belo Horizonte, MG, Brazil
Universidade Federal de São Paulo. Escola Paulista de Medicina. Departamento de Microbiologia, Imunologia e Parasitologia. São Paulo, SP,Brazil
Abstract: Schistosoma mansoni eggs, miracidia and primary sporocysts were labelled with phalloidin-rhodamine to visualize filamentous actin structures. Analysis of these forms by confocal fluorescence microscopy revealed the presence of previously well-defined circular and longitudinal muscle layers. Besides these muscular layers that sustain and provide motility to these parasite forms, we found in these 3 consecutive developmental stages of the parasite previously unidentified actin-rich tubular structures. In the 3 forms, 4 actin-rich tubules could be observed by optical sectioning underneath the well-developed muscle layers. The tubules appear in pairs, transversal to the length of the parasite, and located towards the extremities. By using an anti-flame cell specific antibody we confirmed that the tubules co-localize with flame cells and also determined that the tubule core is filled with microtubules. The additional presence of myosin in these tubules strongly suggests that they are contractile structures
Keywords: Schistosoma mansoni
miracidium
egg
sporocyst
flame cells
Actin
myosin
tubulin
tubule
Issue Date: 2006
Publisher: Cambridge University Press
Citation: BAHIA, Diana et al. The distribution of motor proteins in the muscles and flame cells of the Schistosoma mansoni miracidium and primary sporocyst. Parasitology. 2006 Sep;133(Pt 3):321-9.
ISSN: 0031-1820
Copyright: restricted access
Appears in Collections:MG - IRR - Artigos de Periódicos

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