Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/7163
Title: Relationship between canine visceral leishmaniosis and the Leishmania (Leishmania) chagasi burden in dermal inflammatory foci
Authors: Giunchetti, Rodolfo Cordeiro
Mayrink, Wilson
Genaro, Odair
Carneiro, Cláudia Martins
Corrêa, Rodrigo Oliveira
Martins Filho, Olindo Assis
Marques, Marcos José
Tafuri, Washington Luiz
Reis, Alexandre Barbosa
Affilliation: Universidade Federal de Ouro Preto. Instituto de Ciencias Exatas e Biologicas. Nucleo de Pesquisas em Ciencias Biologicas. Laboratorio de Imunopatologia. Ouro Preto, MG, Brasil/Fundação Oswaldo Cruz. Centro de Pesquisas Rene Rachou. Laboratorio de Imunologia Celular e Molecular. Belo Horizonte, MG, Brasil
Universidade Federal de Minas Gerais. Instituto de Ciencias Biologicas. Departamento de Parasitologia. Laboratorio de Leishmanioses. Belo Horizonte, MG, Brasil
Universidade Federal de Minas Gerais. Instituto de Ciencias Biologicas. Departamento de Parasitologia. Laboratorio de Leishmanioses. Belo Horizonte, MG, Brasil/Universidade Federal de Ouro Preto. Escola de Farmacia. Departamento de Analises Clinicas. Ouro Preto, MG, Brasil
Universidade Federal de Ouro Preto. Instituto de Ciencias Exatas e Biologicas. Nucleo de Pesquisas em Ciencias Biologicas. Laboratorio de Imunopatologia. Ouro Preto, MG, Brasil Universidade Federal de Ouro Preto. Escola de Farmacia. Departamento de Analises Clinicas. Ouro Preto, MG, Brasil
Fundação Oswaldo Cruz. Centro de Pesquisas Rene Rachou. Laboratorio de Imunologia Celular e Molecular. Belo Horizonte, MG, Brasil
Fundação Oswaldo Cruz. Centro de Pesquisas Rene Rachou. Laboratorio de Doença de Chagas. Belo Horizonte, MG, Brasil
Universidade Federal de Minas Gerais. Instituto de Ciencias Biologicas. Departamento de Parasitologia. Laboratorio de Leishmanioses. Belo Horizonte, MG, Brasil
Universidade Federal de Ouro Preto. Instituto de Ciencias Exatas e Biologicas. Nucleo de Pesquisas em Ciencias Biologicas. Laboratorio de Imunopatologia. Ouro Preto, MG, Brasil
Universidade Federal de Ouro Preto. Instituto de Ciencias Exatas e Biologicas. Nucleo de Pesquisas em Ciencias Biologicas. Laboratorio de Imunopatologia. Ouro Preto, MG, Brasil/Fundação Oswaldo Cruz. Centro de Pesquisas Rene Rachou. Laboratorio de Imunologia Celular e Molecular. Belo Horizonte, MG, Brasil
Abstract: The skin is the first point of contact with organisms of the genus Leishmania from sand fly vectors, and apparently normal skin of sick dogs harbours amastigote forms of Leishmania chagasi. In relation to canine visceral leishmaniosis (CVL), the ear skin was examined in 10 uninfected dogs (UDs) and in 31 dogs dogs naturally infected with L. chagasi. The infected animals consisted of 10 symptomless dogs (SLDs), 12 mildly affected dogs (MADs) and nine affected dogs (ADs). A higher parasite burden was demonstrated in ADs than in SLDs by anti-Leishmania immunohistochemistry (P<0.01), and by Leishman Donivan Unit (LDU) indices (P=0.0024) obtained from Giemsa-stained impression smears. Sections stained with haematoxylin and eosin demonstrated a higher intensity of inflammatory changes in ADs than in SLDs (P<0.05), and in the latter group flow cytometry demonstrated a correlation (P=0.05/r=0.7454) between the percentage of CD14(+) monocytes in peripheral blood and chronic dermal inflammation. Extracellular matrix assessment for reticular fibres by staining of sections with Masson trichrome and Gomori ammoniacal silver demonstrated a decrease in collagen type I and an increase in collagen type III as the clinical signs increased. The data on correlation between cellular phenotypes and histological changes seemed to reflect cellular activation and migration from peripheral blood to the skin, mediated by antigenic stimulation. The results suggested that chronic dermal inflammation and cutaneous parasitism were directly related to the severity of clinical disease.
Keywords: dog
kala azar
Leishmania chagasi
parasitic infection
Issue Date: 2006
Publisher: Elsevier
Citation: GIUNCHETTI, Rodolfo Cordeiro et al. Relationship between canine visceral leishmaniosis and the Leishmania (Leishmania) chagasi burden in dermal inflammatory foci. J Comp Pathol. 2006 Aug-Oct;135(2-3):100-7
ISSN: Universidade Federal de Ouro Preto. Instituto de Ciencias Exatas e Biologicas. Nucleo de Pesquisas em Ciencias Biologicas. Laboratorio de Imunopatologia. Ouro Preto, MG, Brasil/Fundação Oswaldo Cruz. Centro de Pesquisas Rene Rachou. Laboratorio de Imunologia Celular e Molecular. Belo Horizonte, MG, Brasil
Copyright: restricted access
Appears in Collections:MG - IRR - Artigos de Periódicos

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